Accession	MF_01648
Dates	17-APR-2008 (Created) 24-JUL-2008 (Last updated, Version 7)

Data class

Protein

Predictors

HAMAP; MF_01648; [distribution of match scores in UniProtKB];[seed alignment for MF_01648]

Identifier

HCAE

Protein name

RecName: Full=3-phenylpropionate/cinnamic acid dioxygenase subunit alpha; EC=1.14.12.19; AltName: Full=Digoxigenin subunit alpha;

Gene name

hcaE

FUNCTION: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively (By similarity).

CATALYTIC ACTIVITY: 3-phenylpropanoic acid + NADH + O(2) = cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD(+).

CATALYTIC ACTIVITY: Cinnamic acid + H(+) + NADH + O(2) = cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + NAD(+).

COFACTOR: Binds 1 iron ion (By similarity).

PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid degradation.

SUBUNIT: This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) (By similarity).

SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.

SIMILARITY: Contains 1 Rieske domain.

PROSITE	PS51296; RIESKE; 1; trigger=PRU00628; PS00570; RING_HYDROXYL_ALPHA; 1;
Pfam	PF00355; Rieske; 1; PF00848; Ring_hydroxyl_A; 1;
PRINTS	PR00090; RNGDIOXGNASE; 1;

2Fe-2S, Aromatic hydrocarbons catabolism, Dioxygenase, Iron, Iron-sulfur, Metal-binding, NAD, Oxidoreductase.

GO:0005506; Molecular function: iron ion binding.
GO:0008695; Molecular function: 3-phenylpropionate dioxygenase activity.
GO:0019439; Biological process: aromatic compound catabolic process.

From: HCAE_ECOLI (P0ABR5)
Key		From		To		Description		Condition		FTGroup
METAL		85		85		Iron-sulfur (2Fe-2S) (By similarity)		C
METAL		87		87		Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity)		H
METAL		105		105		Iron-sulfur (2Fe-2S) (By similarity)		C
METAL		108		108		Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity)		H
METAL		213		213		Iron (By similarity)		H
METAL		218		218		Iron (By similarity)		H

Size range:	453-453 amino acids
Related UniRules:	None
Template:	P0ABR5 (HCAE_ECOLI)
Scope:	Bacteria; Enterobacteriaceae
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None