| Accession |
MF_01631 |
| Dates |
4-APR-2006 (Created)
7-JUN-2008 (Last updated, Version 23) |
| Protein name |
| RecName: |
Full=Bifunctional protein glmU; |
| RecName: |
Full=UDP-N-acetylglucosamine pyrophosphorylase;
EC=2.7.7.23; |
| AltName: |
Full=N-acetylglucosamine-1-phosphate uridyltransferase; |
| RecName: |
Full=Glucosamine-1-phosphate N-acetyltransferase;
EC=2.3.1.157; |
|
FUNCTION: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc (By similarity).
CATALYTIC ACTIVITY: Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.
CATALYTIC ACTIVITY: UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-D-glucosamine.
COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
PATHWAY: Nucleotide-sugar biosynthesis; UDP-N-acetyl-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from D-glucosamine 6-phosphate (route II): step 2/2.
PATHWAY: Nucleotide-sugar biosynthesis; UDP-N-acetyl-D-glucosamine biosynthesis; UDP-N-acetyl-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.
PATHWAY: Context: Lipopolysaccharide (LPS) biosynthesis; lipid A biosynthesis.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.
SIMILARITY: In the C-terminal section; belongs to the transferase hexapeptide repeat family.
Cytoplasm,
Acyltransferase,
Cell shape,
Cell wall biogenesis/degradation,
Magnesium,
Metal-binding,
Multifunctional enzyme,
Nucleotidyltransferase,
Peptidoglycan synthesis,
Repeat,
Transferase.
GO:0000287; Molecular function: magnesium ion binding.
GO:0003977; Molecular function: UDP-N-acetylglucosamine diphosphorylase activity.
GO:0019134; Molecular function: glucosamine-1-phosphate N-acetyltransferase activity.
GO:0000902; Biological process: cell morphogenesis.
GO:0009252; Biological process: peptidoglycan biosynthetic process.
GO:0005737; Cellular component: cytoplasm.
| From: GLMU_ECOLI (P0ACC7) |
| Key |
|
From |
|
To |
|
Description |
|
Condition |
|
FTGroup |
| REGION |
|
Nter |
|
229 |
|
Pyrophosphorylase (By similarity) |
|
|
|
|
| REGION (Optional) |
|
11 |
|
14 |
|
Substrate binding (By similarity) |
|
L-A-A-G |
|
|
| REGION (Optional) |
|
81 |
|
82 |
|
Substrate binding (By similarity) |
|
G-T |
|
|
| REGION |
|
230 |
|
250 |
|
Linker (By similarity) |
|
|
|
|
| REGION |
|
251 |
|
Cter |
|
N-acetyltransferase (By similarity) |
|
|
|
|
| METAL |
|
105 |
|
105 |
|
Magnesium (By similarity) |
|
D |
|
|
| METAL |
|
227 |
|
227 |
|
Magnesium (By similarity) |
|
N |
|
|
| ACT_SITE |
|
363 |
|
363 |
|
Proton acceptor (By similarity) |
|
H |
|
|
| BINDING (Optional) |
|
76 |
|
76 |
|
Substrate (By similarity) |
|
Q |
|
|
| BINDING (Optional) |
|
140 |
|
140 |
|
Substrate; via amide nitrogen (By similarity) |
|
G |
|
|
| BINDING (Optional) |
|
154 |
|
154 |
|
Substrate (By similarity) |
|
E |
|
|
| BINDING (Optional) |
|
169 |
|
169 |
|
Substrate (By similarity) |
|
N |
|
|
| BINDING (Optional) |
|
387 |
|
387 |
|
Acetyl-CoA (By similarity) |
|
Y |
|
|
| BINDING (Optional) |
|
405 |
|
405 |
|
Acetyl-CoA (By similarity) |
|
S |
|
|
| BINDING (Optional) |
|
423 |
|
423 |
|
Acetyl-CoA; via amide nitrogen (By similarity) |
|
[ACGST] |
|
|
| BINDING (Optional) |
|
440 |
|
440 |
|
Acetyl-CoA (By similarity) |
|
R |
|
|
| Size range: |
339-500 amino acids |
| Related UniRules: |
None |
| Template: |
P0ACC7 (GLMU_ECOLI); Q97R46 (GLMU_STRPN): [Recover all] |
| Scope: |
Bacteria |
| Fusion: |
Nter: None; Cter: <Unknown> |
| Duplicate: |
None |
| Plasmid encoded: |
None |
| Comments: |
SYNJA and SYNJB are fusioned in their C-terminal parts to an unknown domain. |
View rule in raw text format (no links)
ExPASy Home page
