Accession	MF_01630
Dates	18-JAN-2006 (Created) 7-JUN-2008 (Last updated, Version 16)

Data class

Protein

Predictors

HAMAP; MF_01630; [distribution of match scores in UniProtKB];[seed alignment for MF_01630]

Identifier

NAPA

Protein name

RecName: Full=Nitrate reductase; EC=1.7.99.4; Flags: Precursor;

Protein name

RecName: Full=Periplasmic nitrate reductase; EC=1.7.99.4; Flags: Precursor;

Gene name

napA

FUNCTION: Catalytic subunit of the nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).

FUNCTION: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).

CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced acceptor.

COFACTOR: Binds 1 4Fe-4S cluster (By similarity).

COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).

COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit (By similarity).

SUBUNIT: Interacts with napB (By similarity).

SUBCELLULAR LOCATION: Secreted (Potential). Note=Membrane-associated (Potential).

SUBCELLULAR LOCATION: Periplasm (By similarity).

PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

PROSITE	PS00551; MOLYBDOPTERIN_PROK_1; 1; PS00490; MOLYBDOPTERIN_PROK_2; 1; PS00932; MOLYBDOPTERIN_PROK_3; 1; PS51318; TAT; 0-1; trigger=PRU00648;
Pfam	PF04879; Molybdop_Fe4S4; 1; PF00384; Molybdopterin; 1; PF01568; Molydop_binding; 1;
TIGRFAMs	TIGR01706; NAPA; 1; TIGR01409; TAT_signal_seq; 0-1;

General

Signal; -; 1; trigger=Yes;

4Fe-4S, Electron transport, Iron, Iron-sulfur, Metal-binding, Molybdenum, Nitrate assimilation, Oxidoreductase.

Periplasm.

Signal, Transport.

GO:0009055; Molecular function: electron carrier activity.
GO:0005506; Molecular function: iron ion binding.
GO:0008940; Molecular function: nitrate reductase activity.
GO:0006777; Biological process: Mo-molybdopterin cofactor biosynthetic process.
GO:0042128; Biological process: nitrate assimilation.
GO:0055114; Biological process: oxidation reduction.

GO:0042597; Cellular component: periplasmic space.

From: NAPA_ECOLI (P33937)
Key		From		To		Description		Condition		FTGroup
METAL		46		46		Iron-sulfur (4Fe-4S) (By similarity)		C
METAL		49		49		Iron-sulfur (4Fe-4S) (By similarity)		C
METAL		53		53		Iron-sulfur (4Fe-4S) (By similarity)		C
METAL		81		81		Iron-sulfur (4Fe-4S) (By similarity)		C

Size range:	754-937 amino acids
Related UniRules:	None
Template:	P33937 (NAPA_ECOLI)
Scope:	Bacteria; Actinobacteria Bacteria; Alphaproteobacteria Bacteria; Betaproteobacteria Bacteria; Deltaproteobacteria Bacteria; Epsilonproteobacteria Bacteria; Gammaproteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in PHOPR
Plasmid encoded:	in RALEU, RHIME, RHOS4