Accession	MF_01629
Dates	14-DEC-2005 (Created) 7-JUN-2008 (Last updated, Version 13)

Data class

Protein

Predictors

HAMAP; MF_01629; [distribution of match scores in UniProtKB];[seed alignment for MF_01629]

Identifier

PDXH

Protein name

RecName: Full=Pyridoxine/pyridoxamine 5'-phosphate oxidase; EC=1.4.3.5; AltName: Full=PNP/PMP oxidase; Short=PNPOx; AltName: Full=Pyridoxal 5'-phosphate synthase;

Gene name

pdxH

FUNCTION: Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) (By similarity).

CATALYTIC ACTIVITY: Pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2).

CATALYTIC ACTIVITY: Pyridoxine 5'-phosphate + O(2) = pyridoxal 5'-phosphate + H(2)O(2).

COFACTOR: Binds 1 FMN per subunit (By similarity).

PATHWAY: Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.

PATHWAY: Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the pyridoxamine 5'-phosphate oxidase family.

PROSITE	PS01064; PYRIDOX_OXIDASE; 1;
Pfam	PF01243; Pyridox_oxidase; 1;
TIGRFAMs	TIGR00558; pdxH; 1;

Flavoprotein, FMN, Oxidoreductase, Pyridoxine biosynthesis.

GO:0004733; Molecular function: pyridoxamine-phosphate oxidase activity.
GO:0010181; Molecular function: FMN binding.
GO:0042816; Biological process: vitamin B6 metabolic process.

From: PDXH_ECOLI (P0AFI7)
Key		From		To		Description		Condition		FTGroup
NP_BIND		82		83		FMN (By similarity)		[YF]-[TS]
NP_BIND		146		147		FMN (By similarity)		Q-S
REGION (Optional)		14		17		Substrate binding (By similarity)		R-x-x-Y
REGION		197		199		Substrate binding (By similarity)		R-x-[HN]
BINDING		67		67		FMN (By similarity)		R
BINDING		70		70		FMN; via amide nitrogen (By similarity)		L
BINDING		72		72		Substrate (By similarity)		[KR]
BINDING		89		89		FMN (By similarity)		K
BINDING		129		129		Substrate (By similarity)		Y
BINDING		133		133		Substrate (By similarity)		R
BINDING (Optional)		137		137		Substrate (By similarity)		S

Size range:	192-267 amino acids
Related UniRules:	None
Template:	P0AFI7 (PDXH_ECOLI); P65682 (PDXH_MYCTU): [Recover all]
Scope:	Bacteria; Actinobacteria Bacteria; Bacteroidetes Bacteria; Cyanobacteria Bacteria; Deinococcus-Thermus Bacteria; Planctomycetes Bacteria; Proteobacteria Bacteria; Spirochaetes
Fusion:	Nter: None; Cter: None
Duplicate:	in RALSO, THICR
Plasmid encoded:	in RALSO