Accession	MF_01321
Dates	6-JAN-2004 (Created) 26-SEP-2008 (Last updated, Version 22)

Data class

Protein

Predictors

HAMAP; MF_01321; [distribution of match scores in UniProtKB];[seed alignment for MF_01321]

Identifier

RPOB

Protein name

RecName: Full=DNA-directed RNA polymerase subunit beta; Short=RNAP subunit beta; EC=2.7.7.6; AltName: Full=Transcriptase subunit beta; AltName: Full=RNA polymerase subunit beta;

Protein name

RecName: Full=DNA-directed RNA polymerase subunit beta; EC=2.7.7.6; AltName: Full=PEP; AltName: Full=Plastid-encoded RNA polymerase subunit beta; Short=RNA polymerase subunit beta;

Gene name

rpoB

FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).

CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).

SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).

SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast.

SIMILARITY: Belongs to the RNA polymerase beta chain family.

Pfam	PF04563; RNA_pol_Rpb2_1; 1; PF04561; RNA_pol_Rpb2_2; 1; PF04565; RNA_pol_Rpb2_3; 1; PF00562; RNA_pol_Rpb2_6; 1; PF04560; RNA_pol_Rpb2_7; 1;
TIGRFAMs	TIGR02013; rpoB; 1;
PROSITE	PS01166; RNA_POL_BETA; 1;

Acetylation.

Transcription, Transferase, Nucleotidyltransferase, DNA-directed RNA polymerase.

GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed RNA polymerase activity.
GO:0006351; Biological process: transcription, DNA-dependent.

GO:0009507; Cellular component: chloroplast.

From: RPOB_ECOLI (P0A8V2)
Key		From		To		Description		Condition		FTGroup
MOD_RES		1022		1022		N6-acetyllysine (By similarity)		K
MOD_RES		1200		1200		N6-acetyllysine (By similarity)		K

Size range:	1060-1499 amino acids
Related UniRules:	None
Template:	Q9KWU7 (RPOB_THEAQ); P0A8V2 (RPOB_ECOLI): [Recover all]
Scope:	Bacteria Plastid
Fusion:	Nter: None; Cter: MF_01322 (rpoC)
Duplicate:	in CLOBH, NOCFA, RHOS1
Plasmid encoded:	in SHEB5
Comments:	Fused with rpoC in Helicobacter species and at least some Wolbachia, but not in other epsilon proteobateria. CHLVU, ODOSI and PSEAK have large inserts, HETCA has a deletion, CHLRE is split. None are used in the alignment.