Accession	MF_01208
Dates	30-MAY-2002 (Created) 31-OCT-2008 (Last updated, Version 23)

Data class

Protein

Predictors

HAMAP; MF_01208_B; [distribution of match scores in UniProtKB];[seed alignment for MF_01208_B]

Predictors

HAMAP; MF_01208_A; [distribution of match scores in UniProtKB];[seed alignment for MF_01208_A]

Identifier

PYRE

Protein name

RecName: Full=Orotate phosphoribosyltransferase; Short=OPRT; Short=OPRTase; EC=2.4.2.10;

Gene name

pyrE

FUNCTION: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) (By similarity).

CATALYTIC ACTIVITY: Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.

COFACTOR: Magnesium (By similarity).

PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 5/6.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Pfam	PF00156; Pribosyltran; 1;
TIGRFAMs	TIGR00336; pyrE; 1;
PROSITE	PS00103; PUR_PYR_PR_TRANSFER; 1;

Pyrimidine biosynthesis, Transferase, Glycosyltransferase, Magnesium.

GO:0000287; Molecular function: magnesium ion binding.
GO:0004588; Molecular function: orotate phosphoribosyltransferase activity.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

From: PYRE_SALTY (P08870)
Key		From		To		Description		Condition		FTGroup
REGION (Optional)		34		35		Orotate binding (By similarity)		F-F
REGION (Optional)		72		73		5-phosphoribose 1-diphosphate binding (By similarity)		Y-K
REGION		124		132		5-phosphoribose 1-diphosphate binding (By similarity)
BINDING (Optional)		26		26		5-phosphoribose 1-diphosphate (By similarity)		[KR]
BINDING (Optional)		99		99		5-phosphoribose 1-diphosphate; shared with dimeric partner (By similarity)		R
BINDING (Optional)		100		100		5-phosphoribose 1-diphosphate (By similarity)		K
BINDING (Optional)		103		103		5-phosphoribose 1-diphosphate; shared with dimeric partner (By similarity)		K
BINDING (Optional)		105		105		5-phosphoribose 1-diphosphate; shared with dimeric partner (By similarity)		H
BINDING (Optional)		128		128		Orotate (By similarity)		[ST]
BINDING (Optional)		156		156		Orotate (By similarity)		R

From: PYRE_PYRHO (O58855)
Key		From		To		Description		Condition		FTGroup
REGION		119		127		5-phosphoribose 1-diphosphate binding (By similarity)
BINDING (Optional)		93		93		5-phosphoribose 1-diphosphate; shared with dimeric partner (By similarity)		R
BINDING (Optional)		94		94		5-phosphoribose 1-diphosphate (By similarity)		K
BINDING (Optional)		97		97		5-phosphoribose 1-diphosphate; shared with dimeric partner (By similarity)		[KR]
BINDING (Optional)		99		99		5-phosphoribose 1-diphosphate; shared with dimeric partner (By similarity)		H
BINDING (Optional)		123		123		Orotate (By similarity)		T
BINDING (Optional)		151		151		Orotate (By similarity)		R

Size range:

179-237 amino acids

Size range:

166-203 amino acids

Related UniRules:	MF_01214 (PYREL)
Template:	P0A7E3 (PYRE_ECOLI); P08870 (PYRE_SALTY); P42719 (PYRE_RHILT); P61498 (PYRE_THET2); P46534 (PYRE_BACCL): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: MF_01200 (pyrF); Cter: None
Duplicate:	in ANASP, RHILO
Plasmid encoded:	None