Accession	MF_01161
Dates	1-MAR-2005 (Created) 29-JUL-2008 (Last updated, Version 18)

Data class

Protein

Predictors

HAMAP; MF_01161; [distribution of match scores in UniProtKB];[seed alignment for MF_01161]

Identifier

TILS

Protein name

RecName: Full=tRNA(Ile)-lysidine synthase; EC=6.3.4.-; AltName: Full=tRNA(Ile)-lysidine synthetase; AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase;

Protein name

RecName: Full=tRNA(Ile)-lysidine synthase, chloroplastic; EC=6.3.4.-; AltName: Full=tRNA(Ile)-lysidine synthetase; AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase;

Gene name

tilS

FUNCTION: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast.

DOMAIN: The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

SIMILARITY: Belongs to the tRNA(Ile)-lysidine synthase family.

Pfam	PF01171; ATP_bind_3; 1;

ATP-binding, Nucleotide-binding.

Cytoplasm.

Ligase, tRNA processing.

GO:0005524; Molecular function: ATP binding.

GO:0016879; Molecular function: ligase activity, forming carbon-nitrogen bonds.
GO:0006400; Biological process: tRNA modification.

GO:0005737; Cellular component: cytoplasm.

GO:0009507; Cellular component: chloroplast.

From: TILS_ECOLI (P52097)
Key		From		To		Description		Condition		FTGroup
NP_BIND		20		25		ATP (Potential)		S-G-G-x-D-S

Size range:	278-648 amino acids
Related UniRules:	None
Template:	P52097 (TILS_ECOLI)
Scope:	Bacteria Plastid
Fusion:	Nter: None; Cter: <hprT>, <CMP/dCMP deaminase zinc-binding domain>
Duplicate:	None
Plasmid encoded:	None