HAMAP family: MF

General rule information

Accession	MF_01035
Dates	1-OCT-2002 (Created) 10-OCT-2008 (Last updated, Version 22)

Data class

Protein

Predictors

HAMAP; MF_01035; [distribution of match scores in UniProtKB];[seed alignment for MF_01035]

Propagated annotation

Identifier, protein and gene names

Identifier

LEU3

Protein name

RecName:	Full=3-isopropylmalate dehydrogenase; EC=1.1.1.85;
AltName:	Full=Beta-IPM dehydrogenase; Short=IMDH;
AltName:	Full=3-IPM-DH;

Gene name

leuB

Comments

FUNCTION: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity).

CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.

COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily.

Cross-references

Pfam	PF00180; isodh; 1;
PROSITE	PS00470; IDH_IMDH; 1;

Keywords

Cytoplasm, Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis, Magnesium, Manganese, Metal-binding, NAD, Oxidoreductase.

Gene Ontology

GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Features

`From: LEU3_MYCTU (P95313)`
`Key`	`From`	`To`	`Description`	`Condition`	`FTGroup`
`NP_BIND`	`271`	`283`	`NAD (By similarity)`	`G-S-A-P-D-I-x(5)-A-[DN]`

`METAL`	`211`	`211`	`Magnesium or manganese (By similarity)`	`D`	`1`
`METAL`	`235`	`235`	`Magnesium or manganese (By similarity)`	`D`	`1`
`METAL`	`239`	`239`	`Magnesium or manganese (By similarity)`	`D`	`1`

`BINDING`	`87`	`87`	`Substrate (By similarity)`	`R`
`BINDING`	`97`	`97`	`Substrate (By similarity)`	`R`
`BINDING`	`121`	`121`	`Substrate (By similarity)`	`R`
`BINDING`	`211`	`211`	`Substrate (By similarity)`	`D`
`SITE`	`128`	`128`	`Important for catalysis (By similarity)`	`Y`
`SITE`	`178`	`178`	`Important for catalysis (By similarity)`	`K`

Additional information

Size range:	335-357 amino acids
Related UniRules:	MF_01033 (LEU3)
Template:	P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_THIFE); Q5SIY4 (LEU3_THET8): [Recover all]
Scope:	Bacteria; Actinobacteridae
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	See MF_01033 for other leuB

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