Accession	MF_00379
Dates	1-JUN-2001 (Created) 23-MAR-2009 (Last updated, Version 22)

Data class

Protein

Predictors

HAMAP; MF_00379; [distribution of match scores in UniProtKB];[seed alignment for MF_00379]

Identifier

MNME

Protein name

RecName: Full=tRNA modification GTPase mnmE; EC=3.6.-.-;

Gene name

mnmE, trmE

Protein name

RecName: Full=Probable tRNA modification GTPase mnmE; EC=3.6.-.-;

Gene name

mnmE, thdF

FUNCTION: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 (By similarity).

COFACTOR: Binds 1 potassium ion per subunit (By similarity).

SUBUNIT: Homodimer (By similarity). Heterotetramer of two mnmE and two mnmG subunits (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast.

SIMILARITY: Belongs to the era/mnmE GTP-binding protein family. MnmE subfamily.

Pfam	PF01926; MMR_HSR1; 1;
PRINTS	PR00326; GTP1OBG; 1;
TIGRFAMs	TIGR00650; MG442; 1; TIGR00450; mnmE_trmE_thdF; 1; TIGR00231; small_GTP; 1;

Cytoplasm.

GTP-binding, Hydrolase, Magnesium, Metal-binding, Nucleotide-binding, Potassium, tRNA processing.

GO:0005525; Molecular function: GTP binding.
GO:0003924; Molecular function: GTPase activity.
GO:0006400; Biological process: tRNA modification.

GO:0005737; Cellular component: cytoplasm.

GO:0009507; Cellular component: chloroplast.

From: MNME_ECOLI (P25522)
Key		From		To		Description		Condition		FTGroup
NP_BIND		226		231		GTP (By similarity)		N-[VSAT]-G-K-S-[TSQR]
NP_BIND		245		251		GTP (By similarity)		[TSMAD]-x(3)-G-[TSR]-T
NP_BIND		270		273		GTP (By similarity)		D-[TS]-A-G
NP_BIND (Optional)		335		338		GTP (By similarity)		N-K-A-D
NP_BIND (Optional)		358		360		GTP (By similarity)		S-A-R
METAL (Optional)		226		226		Potassium (By similarity)		N		1
METAL		230		230		Magnesium (By similarity)		S
METAL (Optional)		245		245		Potassium; via carbonyl oxygen (By similarity)		[TS]		1
METAL (Optional)		247		247		Potassium; via carbonyl oxygen (By similarity)		[IVL]		1
METAL (Optional)		250		250		Potassium (By similarity)		[TS]		1
METAL		251		251		Magnesium (By similarity)		T
BINDING		23		23		Formyltetrahydrofolate (By similarity)		[RK]		2
BINDING		80		80		Formyltetrahydrofolate (By similarity)		E		2
BINDING		120		120		Formyltetrahydrofolate (By similarity)		[RKVM]		2
BINDING		454		454		Formyltetrahydrofolate (By similarity)		K		2

Size range:	419-550 amino acids
Related UniRules:	None
Template:	P25522 (MNME_ECOLI); Q9WYA4 (MNME_THEMA): [Recover all]
Scope:	Bacteria Plastid
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None