Accession	MF_00235
Dates	1-JUN-2001 (Created) 26-SEP-2008 (Last updated, Version 30)

Data class

Protein

Predictors

HAMAP; MF_00235; [distribution of match scores in UniProtKB];[seed alignment for MF_00235]

Identifier

KAD

Protein name

RecName: Full=Adenylate kinase; Short=AK; EC=2.7.4.3; AltName: Full=ATP-AMP transphosphorylase;

Gene name

adk

FUNCTION: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in the energy metabolism and nucleotide synthesis, is essential for maintenance and cell growth (By similarity).

CATALYTIC ACTIVITY: ATP + AMP = 2 ADP.

PATHWAY: Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

DOMAIN: Consists of three domains, a large central CORE domain and two small peripheral domains, AMP binding and LID. The LID domain closes over the site of phosphoryl transfer upon ATP binding (By similarity).

DOMAIN: Consists of three domains, a large central CORE domain and two small peripheral domains, AMP binding and LID. The LID domain is a solvent-exposed domain that is much shorter in Mycobacterium than in many other bacteria like E.coli, in which it closes over the site of phosphoryl transfer upon ATP binding. The AMP binding domain seems to play an important role in the catalysis and structural stability of the protein (By similarity).

MISCELLANEOUS: The zinc ion does not participate in catalysis. It has a structural role in stabilizing the LID domain, which does not seem to be involved in directly binding DNA/RNA (By similarity).

SIMILARITY: Belongs to the adenylate kinase family.

Pfam	PF00406; adenylatekinase; 1;
PRINTS	PR00094; ADENYLTKNASE; 1;
TIGRFAMs	TIGR01351; adk; 1;
PROSITE	PS00113; ADENYLATE_KINASE; 1;

Acetylation.

ATP-binding, Cytoplasm, Transferase, Kinase, Nucleotide-binding, Nucleotide biosynthesis.

Metal-binding, Zinc.

GO:0004017; Molecular function: adenylate kinase activity.
GO:0005524; Molecular function: ATP binding.

GO:0008270; Molecular function: zinc ion binding.

GO:0009165; Biological process: nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: KAD_ECOLI (P69441)
Key		From		To		Description		Condition		FTGroup
NP_BIND		7		15		ATP (By similarity)		G-x-[NP]-[GA]-x-G-K-[GST]-[ST]
NP_BIND		31		59		AMP (By similarity)

Key		From		To		Description		Condition		FTGroup
REGION		122		159		LID

Key		From		To		Description		Condition		FTGroup
MOD_RES		192		192		N6-acetyllysine (By similarity)		K

From: KAD_MYCTU (P69440)
Key		From		To		Description		Condition		FTGroup
REGION		125		134		LID

From: KAD_BACSU (P16304)
Key		From		To		Description		Condition		FTGroup
METAL (Optional)		130		130		Zinc (By similarity)		C		1
METAL (Optional)		133		133		Zinc (By similarity)		C		1
METAL (Optional)		150		150		Zinc (By similarity)		C		1
METAL (Optional)		153		153		Zinc (By similarity)		[CD]		1

Key		From		To		Description		Condition		FTGroup
REGION		128		159		LID

Size range:	181-253 amino acids
Related UniRules:	None
Template:	P69441 (KAD_ECOLI); P69440 (KAD_MYCTU); P16304 (KAD_BACSU); P27142 (KAD_BACST); P84139 (KAD_BACGO): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in ANASP, SYNY3
Plasmid encoded:	None
Comments:	FT condition for ATP binding is not fulfilled in the second copy of adk in ANASP; not shown in alignment