Accession	MF_00191
Dates	1-JUN-2001 (Created) 7-JUN-2008 (Last updated, Version 30)

Data class

Protein

Predictors

HAMAP; MF_00191; [distribution of match scores in UniProtKB];[seed alignment for MF_00191]

Identifier

ISPH

Protein name

RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase; EC=1.17.1.2;

Gene name

ispH

FUNCTION: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) (By similarity).

CATALYTIC ACTIVITY: Isopentenyl diphosphate + NAD(P)(+) + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.

CATALYTIC ACTIVITY: Dimethylallyl diphosphate + NAD(P)(+) + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.

COFACTOR: Binds 1 3Fe-4S cluster per subunit (By similarity).

COFACTOR: Binds 1 3Fe-4S cluster (By similarity).

PATHWAY: Isoprenoid biosynthesis; dimethylallyl-PP biosynthesis; dimethylallyl-PP from 1-hydroxy-2-methyl-2-butenyl 4-diphosphate: step 1/1.

PATHWAY: Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the ispH family.

Pfam	PF02401; LYTB; 1;
TIGRFAMs	TIGR00216; ispH_lytB; 1;

3Fe-4S, Iron, Iron-sulfur, Isoprene biosynthesis, Metal-binding, NADP, Oxidoreductase.

GO:0051745; Molecular function: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity.
GO:0019288; Biological process: isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway.
GO:0016114; Biological process: terpenoid biosynthetic process.

Size range:	273-417 amino acids
Related UniRules:	None
Template:	P62623 (ISPH_ECOLI); O67625 (ISPH_AQUAE); Q55643 (ISPH_SYNY3): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: <S1>
Duplicate:	in BRAJA, MYCBO, MYCTU, RHOPA
Plasmid encoded:	None