Accession	MF_00181
Dates	1-JUN-2001 (Created) 21-AUG-2008 (Last updated, Version 27)

Data class

Protein

Predictors

HAMAP; MF_00181; [distribution of match scores in UniProtKB];[seed alignment for MF_00181]

Identifier

AMPA

Protein name

RecName: Full=Probable cytosol aminopeptidase; EC=3.4.11.1; AltName: Full=Leucine aminopeptidase; Short=LAP; AltName: Full=Leucyl aminopeptidase;

Gene name

pepA

FUNCTION: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).

CATALYTIC ACTIVITY: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

COFACTOR: Binds 2 manganese ions per subunit (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the peptidase M17 family.

Pfam	PF00883; Peptidase_M17; 1;
PRINTS	PR00481; LAMNOPPTDASE; 1;
PROSITE	PS00631; CYTOSOL_AP; 1;

Cytoplasm, Hydrolase, Protease, Aminopeptidase, Manganese, Metal-binding.

GO:0004177; Molecular function: aminopeptidase activity.
GO:0008235; Molecular function: metalloexopeptidase activity.
GO:0030145; Molecular function: manganese ion binding.
GO:0005737; Cellular component: cytoplasm.

From: AMPA_ECOLI (P68767)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		282		282		Potential		K
ACT_SITE		356		356		Potential		R
METAL		270		270		Manganese 2 (By similarity)		K
METAL		275		275		Manganese 1 (By similarity)		D
METAL		275		275		Manganese 2 (By similarity)		D
METAL		293		293		Manganese 2 (By similarity)		D
METAL		352		352		Manganese 1 (By similarity)		D
METAL		354		354		Manganese 1 (By similarity)		E
METAL		354		354		Manganese 2 (By similarity)		E

Size range:	445-524 amino acids
Related UniRules:	MF_00504 (PEPB)
Template:	P68767 (AMPA_ECOLI); P27888 (AMPA_RICPR): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in SHEON
Plasmid encoded:	None
Comments:	Possible wrong C-terminal sequence in MYCSA