HAMAP family: MF

General rule information

Accession	MF_00109
Dates	1-JUN-2001 (Created) 26-SEP-2008 (Last updated, Version 31)

Data class

Protein

Predictors

HAMAP; MF_00109; [distribution of match scores in UniProtKB];[seed alignment for MF_00109]

Propagated annotation

Identifier, protein and gene names

Identifier

AROK

case not <OC:Enterobacteriaceae>

Protein name

RecName:

Full=Shikimate kinase;
Short=SK;
EC=2.7.1.71;

end case

case <OC:Enterobacteriaceae>

Protein name

RecName:

Full=Shikimate kinase 1;
Short=SK 1;
EC=2.7.1.71;

end case

Gene name

aroK

Comments

FUNCTION: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate (By similarity).

CATALYTIC ACTIVITY: ATP + shikimate = ADP + shikimate 3-phosphate.

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 5/7.

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the shikimate kinase family.

Cross-references

Pfam	PF01202; SKI; 1;
PRINTS	PR01100; SHIKIMTKNASE; 1;
PROSITE	PS01128; SHIKIMATE_KINASE; 1;

Keywords

Cytoplasm, Amino-acid biosynthesis, Aromatic amino acid biosynthesis, ATP-binding, Kinase, Magnesium, Metal-binding, Nucleotide-binding, Transferase.

Gene Ontology

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004765; Molecular function: shikimate kinase activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Features

`From: AROK_MYCTU (P0A4Z2)`
`Key`	`From`	`To`	`Description`	`Condition`	`FTGroup`
`NP_BIND`	`12`	`17`	`ATP (By similarity)`
`METAL`	`16`	`16`	`Magnesium (By similarity)`	`[ST]`
`BINDING`	`34`	`34`	`Substrate (By similarity)`	`D`
`BINDING`	`58`	`58`	`Substrate (By similarity)`	`R`
`BINDING`	`80`	`80`	`Substrate; via amide nitrogen (By similarity)`	`G`
`BINDING`	`117`	`117`	`ATP (By similarity)`	`[RK]`
`BINDING`	`136`	`136`	`Substrate (By similarity)`	`R`
`BINDING (Optional)`	`153`	`153`	`ATP (By similarity)`	`[RQ]`

Additional information

Size range:	161-214 amino acids
Related UniRules:	MF_01269 (AROL (supersedes the current rule))
Template:	P0A6D7 (AROK_ECOLI); P0A4Z2 (AROK_MYCTU): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: MF_00110 (aroB)
Duplicate:	None
Plasmid encoded:	None
Comments:	Archaeal aroK belong to a separate family (MF_00370). Enterobacteriaceae (except WIGBR, BLOBP, BLOFL, BUCAI, BUCAP and BUCBP) possess a second shikimate kinase (aroL, MF_01269). Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain. Strange longer C-terminus in FRASC; sequence not included in size range.

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