Accession	MF_00061
Dates	1-JUN-2001 (Created) 7-JUN-2008 (Last updated, Version 23)

Data class

Protein

Predictors

HAMAP; MF_00061; [distribution of match scores in UniProtKB];[seed alignment for MF_00061]

Identifier

ISPE

Protein name

RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; Short=CMK; EC=2.7.1.148; AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase;

Gene name

ispE

FUNCTION: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (By similarity).

CATALYTIC ACTIVITY: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.

PATHWAY: Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 3/6.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the GHMP kinase family. IspE subfamily.

Pfam	PF00288; GHMP_kinases; 1;
TIGRFAMs	TIGR00154; ispE; 1;

ATP-binding, Nucleotide-binding, Isoprene biosynthesis, Kinase, Transferase.

GO:0050515; Molecular function: 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity.
GO:0005524; Molecular function: ATP binding.
GO:0016114; Biological process: terpenoid biosynthetic process.

From: ISPE_ECOL6 (Q8FI04)
Key		From		To		Description		Condition		FTGroup
NP_BIND		99		109		ATP (Potential)		[PL]-x-x-[GSA]-G-[LIMFV]-[GA]-[GS]-[GAD]-S-[STAG]
ACT_SITE		10		10		By similarity		K
ACT_SITE		141		141		By similarity		D

Size range:	255-329 amino acids
Related UniRules:	None
Template:	P62615 (ISPE_ECOLI); Q8FI04 (ISPE_ECOL6); P83700 (ISPE_THET8): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	In CHLPN there is a remnant of an ispE gene but which is interrupted by a probable natural frameshift (all CHLPN strains have it). It is orf CP0905