Accession	MF_00059
Dates	25-OCT-2002 (Created) 7-JUN-2008 (Last updated, Version 36)

Data class

Protein

Predictors

HAMAP; MF_00059; [distribution of match scores in UniProtKB];[seed alignment for MF_00059]

Identifier

RPOA

Protein name

RecName: Full=DNA-directed RNA polymerase subunit alpha; Short=RNAP subunit alpha; EC=2.7.7.6; AltName: Full=Transcriptase subunit alpha; AltName: Full=RNA polymerase subunit alpha;

Protein name

RecName: Full=DNA-directed RNA polymerase subunit alpha; Short=PEP; EC=2.7.7.6; AltName: Full=Plastid-encoded RNA polymerase subunit alpha; Short=RNA polymerase subunit alpha;

Gene name

rpoA

FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).

CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).

SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).

SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast.

DOMAIN: The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements (By similarity).

SIMILARITY: Belongs to the RNA polymerase alpha chain family.

Pfam	PF01000; RNA_pol_A_bac; 1; PF03118; RNA_pol_A_CTD; 1;

Transferase, Nucleotidyltransferase, Transcription, DNA-directed RNA polymerase.

GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed RNA polymerase activity.
GO:0006351; Biological process: transcription, DNA-dependent.

GO:0009507; Cellular component: chloroplast.

From: RPOA_ECOLI (P0A7Z4)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		235		Alpha N-terminal domain (alpha-NTD) (By similarity)
REGION		249		Cter		Alpha C-terminal domain (alpha-CTD) (By similarity)

Size range:	309-383 amino acids
Related UniRules:	None
Template:	P0A7Z4 (RPOA_ECOLI); Q925Z2 (RPOA_RHIME); Q9KWU8 (RPOA_THEAQ); Q9Z9H6 (RPOA_THETH): [Recover all]
Scope:	Bacteria Plastid
Fusion:	Nter: None; Cter: None
Duplicate:	in FRATF, FRATH, FRATO, FRATN, FRATT, FRAT1, FRATW, LEPBL, PSYIN, STRAW
Plasmid encoded:	None
Comments:	Divergent BIGNA, CARRP, CHLRE, CHLVU, HELSJ, NEPOL, ONYPE, OLTVI, PELHO, PSEAK, SCEOB, STIHE and Euglids not used in alignment; they are either quite short or have inserts. In PELHO there are 3 rpoA-like genes, but it is not clear if any of them are functional.