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| HAMAP annotation rule: MF_01690 |
| Accession | MF_01690 |
| Dates | 22-APR-2009 (Created) 25-NOV-2009 (Last updated, Version 5) |
| Data class | Protein |
| Predictors | HAMAP; MF_01690; [distribution of match scores in UniProtKB];[seed alignment for MF_01690] |
| Names | DapE |
| Identifier | DAPE |
| Protein name |
|
| Gene name | dapE |
FUNCTION: Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls (By similarity).
CATALYTIC ACTIVITY: N-succinyl-LL-2,6-diaminoheptanedioate + H(2)O = succinate + LL-2,6-diaminoheptanedioate.
COFACTOR: Binds 1 Zn(2+) ion per subunit (By similarity).
COFACTOR: Binds 1 Co(2+) ion per subunit (By similarity).
PATHWAY: Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the peptidase M20A family. DapE subfamily.
| PROSITE |
PS00758; ARGE_DAPE_CPG2_1; 1; PS00759; ARGE_DAPE_CPG2_2; 1; |
| Pfam |
PF07687; M20_dimer; 1; PF01546; Peptidase_M20; 1; |
| TIGRFAMs |
TIGR01246; dapE_proteo; 1; TIGR01910; DapE-ArgE; 1; |
Amino-acid biosynthesis,
Cobalt,
Diaminopimelate biosynthesis,
Hydrolase,
Lysine biosynthesis,
Metal-binding,
Zinc.
GO:0008270; Molecular function: zinc ion binding.
GO:0009014; Molecular function: succinyl-diaminopimelate desuccinylase activity.
GO:0050897; Molecular function: cobalt ion binding.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0019877; Biological process: diaminopimelate biosynthetic process.
GO:0009014; Molecular function: succinyl-diaminopimelate desuccinylase activity.
GO:0050897; Molecular function: cobalt ion binding.
GO:0009089; Biological process: lysine biosynthetic process via diaminopimelate.
GO:0019877; Biological process: diaminopimelate biosynthetic process.
| From: DAPE_ECOLI (P0AED7) | ||||||||||
| Key | From | To | Description | Condition | FTGroup | |||||
| ACT_SITE | 68 | 68 | By similarity | D | ||||||
| ACT_SITE | 133 | 133 | Proton acceptor (By similarity) | E | ||||||
| METAL | 66 | 66 | Cobalt or zinc 1 (By similarity) | H | ||||||
| METAL | 99 | 99 | Cobalt or zinc 1 (By similarity) | D | ||||||
| METAL | 99 | 99 | Cobalt or zinc 2 (By similarity) | D | ||||||
| METAL | 134 | 134 | Cobalt or zinc 2 (By similarity) | E | ||||||
| METAL | 162 | 162 | Cobalt or zinc 1 (By similarity) | E | ||||||
| METAL | 348 | 348 | Cobalt or zinc 2 (By similarity) | H | ||||||
| Size range: | 363-425 amino acids |
| Related UniRules: | None |
| Template: | Q8ZN75 (DAPE_SALTY); P44514 (DAPE_HAEIN); P0AED7 (DAPE_ECOLI): [Recover all] |
| Scope: | Bacteria; Proteobacteria |
| Fusion: | Nter: None; Cter: None |
| Duplicate: | in ALTMD, SHELP, SILST |
| Plasmid encoded: | in PSESY, SILST |
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