ExPASy Proteomics Server

Accession	MF_01621
Dates	18-NOV-2005 (Created) 25-NOV-2009 (Last updated, Version 16)

Data class

Protein

Predictors

HAMAP; MF_01621; [distribution of match scores in UniProtKB];[seed alignment for MF_01621]

Names

FadB

Identifier

FADB

Protein name

RecName: Full=Fatty acid oxidation complex subunit alpha; RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase; EC=4.2.1.17; EC=5.3.3.8; EC=5.1.2.3; RecName: Full=3-hydroxyacyl-CoA dehydrogenase; EC=1.1.1.35;

Gene name

fadB

FUNCTION: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities (By similarity).

CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.

CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O.

CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.

CATALYTIC ACTIVITY: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.

PATHWAY: Lipid metabolism; fatty acid beta-oxidation.

SUBUNIT: Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) (By similarity).

SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

SIMILARITY: In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

PROSITE	PS00067; 3HCDH; 1; PS00166; ENOYL_COA_HYDRATASE; 1;
Pfam	PF00725; 3HCDH; 1; PF02737; 3HCDH_N; 1; PF00378; ECH; 1;
TIGRFAMs	TIGR02437; FadB; 1;

Fatty acid metabolism, Isomerase, Lipid degradation, Lipid metabolism, Lyase, Multifunctional enzyme, NAD, Oxidoreductase.

GO:0003857; Molecular function: 3-hydroxyacyl-CoA dehydrogenase activity.
GO:0004165; Molecular function: dodecenoyl-CoA delta-isomerase activity.
GO:0004300; Molecular function: enoyl-CoA hydratase activity.
GO:0008692; Molecular function: 3-hydroxybutyryl-CoA epimerase activity.
GO:0006631; Biological process: fatty acid metabolic process.
GO:0016042; Biological process: lipid catabolic process.

From: FADB_PSEFR (P28793)
Key		From		To		Description		Condition		FTGroup
NP_BIND		401		403		NAD (By similarity)		[VI]-[VT]-E
NP_BIND		428		430		NAD (By similarity)		N-T-S
REGION		Nter		190		Enoyl-CoA hydratase/isomerase (By similarity)
REGION		312		Cter		3-hydroxyacyl-CoA dehydrogenase (By similarity)
BINDING		297		297		Substrate (By similarity)		D
BINDING		325		325		NAD; via amide nitrogen (By similarity)		M
BINDING		344		344		NAD (By similarity)		D
BINDING		408		408		NAD (By similarity)		K
BINDING		454		454		NAD (By similarity)		N
BINDING		501		501		Substrate (By similarity)		N
BINDING (Optional)		660		660		Substrate (By similarity)		Y
SITE		120		120		Important for catalytic activity (By similarity)		E
SITE		140		140		Important for catalytic activity (By similarity)		E

Size range:	715-729 amino acids
Related UniRules:	None
Template:	P21177 (FADB_ECOLI); P28793 (FADB_PSEFR): [Recover all]
Scope:	Bacteria; Gammaproteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None