ExPASy Proteomics Server

Accession	MF_01601
Dates	25-OCT-2004 (Created) 25-NOV-2009 (Last updated, Version 15)

Data class

Protein

Predictors

HAMAP; MF_01601; [distribution of match scores in UniProtKB];[seed alignment for MF_01601]

Names

Heptose_epimerase

Identifier

HLDD

Protein name

RecName: Full=ADP-L-glycero-D-manno-heptose-6-epimerase; EC=5.1.3.20; AltName: Full=ADP-L-glycero-beta-D-manno-heptose-6-epimerase; Short=ADP-glyceromanno-heptose 6-epimerase; Short=ADP-hep 6-epimerase; Short=AGME;

Gene name

hldD

FUNCTION: Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose (By similarity).

CATALYTIC ACTIVITY: ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose.

COFACTOR: Binds 1 NADP(+) per subunit (By similarity).

PATHWAY: Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 4/4.

SUBUNIT: Homopentamer (By similarity).

DOMAIN: Contains a large N-terminal NADP-binding domain, and a smaller C-terminal substrate-binding domain (By similarity).

SIMILARITY: Belongs to the sugar epimerase family. HldD subfamily.

Pfam	PF01370; Epimerase; 1;

Acetylation.

Carbohydrate metabolism, Isomerase, NADP.

GO:0008712; Molecular function: ADP-glyceromanno-heptose 6-epimerase activity.
GO:0005975; Biological process: carbohydrate metabolic process.

From: HLDD_ECOLI (P67910)
Key		From		To		Description		Condition		FTGroup
NP_BIND		10		11		NADP (By similarity)
NP_BIND		31		32		NADP (By similarity)
NP_BIND		75		79		NADP (By similarity)
REGION		201		204		Substrate binding (By similarity)
ACT_SITE		140		140		Proton acceptor (By similarity)		Y
ACT_SITE		178		178		Proton acceptor (By similarity)		K
BINDING		38		38		NADP (By similarity)		[KQR]
BINDING		53		53		NADP (By similarity)		[KQR]
BINDING		92		92		NADP (By similarity)		N
BINDING		144		144		NADP (By similarity)		K
BINDING		169		169		Substrate (By similarity)		N
BINDING		170		170		NADP; via amide nitrogen (By similarity)		[VI]
BINDING		178		178		NADP (By similarity)		K
BINDING		180		180		Substrate; via carbonyl oxygen (By similarity)		x
BINDING		187		187		Substrate (By similarity)		[HKQ]
BINDING		209		209		Substrate (By similarity)		[HR]
BINDING		272		272		Substrate (By similarity)		Y

Key		From		To		Description		Condition		FTGroup
MOD_RES		267		267		N6-acetyllysine (By similarity)		K

Size range:	291-340 amino acids
Related UniRules:	None
Template:	P67910 (HLDD_ECOLI)
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None