ExPASy Proteomics Server

Accession	MF_01323
Dates	6-JAN-2004 (Created) 25-NOV-2009 (Last updated, Version 21)

Data class

Protein

Predictors

HAMAP; MF_01323; [distribution of match scores in UniProtKB];[seed alignment for MF_01323]

Names

RNApol_bact_RpoC1

Identifier

RPOC1

Protein name

RecName: Full=DNA-directed RNA polymerase subunit gamma; Short=RNAP subunit gamma; EC=2.7.7.6; AltName: Full=Transcriptase subunit gamma; AltName: Full=RNA polymerase subunit gamma;

Protein name

RecName: Full=DNA-directed RNA polymerase subunit beta'; EC=2.7.7.6; AltName: Full=PEP; AltName: Full=Plastid-encoded RNA polymerase subunit beta'; Short=RNA polymerase subunit beta';

Gene name

rpoC1

FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).

CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).

SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast.

SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC1 subfamily.

Pfam	PF04997; RNA_pol_Rpb1_1; 1; PF00623; RNA_pol_Rpb1_2; 1; PF04983; RNA_pol_Rpb1_3; 1; PF05000; RNA_pol_Rpb1_4; 1; PF04998; RNA_pol_Rpb1_5; 1;

Transcription, Transferase, Nucleotidyltransferase, DNA-directed RNA polymerase.

GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed RNA polymerase activity.
GO:0006351; Biological process: transcription, DNA-dependent.

GO:0009507; Cellular component: chloroplast.

Size range:	575-747 amino acids
Related UniRules:	MF_01322 (RPOC (supersedes the current rule))
Template:	P22704 (RPOC1_ANASP); P11705 (RPOC1_SPIOL): [Recover all]
Scope:	Bacteria; Cyanobacteria Plastid
Fusion:	Nter: None; Cter: MF_01324 (rpoC2)
Duplicate:	None
Plasmid encoded:	None
Comments:	In cyanobacteria and plastids, there are two ORFs (this one, rpoC1, MF_01323 and rpoC2, MF_01324) that together are equivalent to the beta' subunit found in all other bacteria (rpoC, MF_01322). However,in the red algae CYAME the rpoC1 and rpoC2 genes are fused into one ORF. This seems to be the result of evolution acting to reduce protein size rather than horizontal transfer from a bacteria. Some algal proteins with inserts and C.reinhardtii (where this protein is split in 2) are considered to be atypical.