ExPASy Proteomics Server

Accession	MF_01293
Dates	5-NOV-2008 (Created) 25-NOV-2009 (Last updated, Version 2)

Data class

Protein

Predictors

HAMAP; MF_01293; [distribution of match scores in UniProtKB];[seed alignment for MF_01293]

Names

TagBP_aldolase_KbaY

Identifier

KBAY

Protein name

RecName: Full=D-tagatose-1,6-bisphosphate aldolase subunit kbaY; Short=TagBP aldolase; Short=TBPA; EC=4.1.2.40; AltName: Full=Tagatose-bisphosphate aldolase; AltName: Full=D-tagatose-bisphosphate aldolase class II; AltName: Full=Ketose 1,6-bisphosphate aldolase class II;

Gene name

kbaY

FUNCTION: Catalytic subunit of the tagatose-1,6-bisphosphate aldolase kbaYZ, which catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6-bisphosphate (TBP). Requires kbaZ subunit for full activity and stability (By similarity).

CATALYTIC ACTIVITY: D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

COFACTOR: Binds 1 zinc ion per subunit (By similarity).

PATHWAY: Carbohydrate metabolism; D-tagatose 6-phosphate degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-phosphate: step 2/2.

SUBUNIT: Homotetramer. Forms a complex with kbaZ (By similarity).

SIMILARITY: Belongs to the class II fructose-bisphosphate aldolase family. TagBP aldolase kbaY subfamily.

PROSITE	PS00602; ALDOLASE_CLASS_II_1; 1; PS00806; ALDOLASE_CLASS_II_2; 1;
Pfam	PF01116; F_bP_aldolase; 1;
TIGRFAMs	TIGR00167; cbbA; 1; TIGR01858; tag_bisphos_ald; 1;

Lyase.

Metal-binding, Zinc.

GO:0008270; Molecular function: zinc ion binding.

GO:0009025; Molecular function: tagatose-bisphosphate aldolase activity.
GO:0005975; Biological process: carbohydrate metabolic process.

From: KBAY_ECOLI (P0AB74)
Key		From		To		Description		Condition		FTGroup
REGION		230		233		Dihydroxyacetone phosphate binding (By similarity)		N-x-A-T
REGION		209		211		Dihydroxyacetone phosphate binding (By similarity)		G-x-S
ACT_SITE		82		82		Proton donor (By similarity)		D
METAL		83		83		Zinc; catalytic (By similarity)		H		1
METAL		180		180		Zinc; catalytic (By similarity)		H		1
METAL		208		208		Zinc; catalytic (By similarity)		H		1
BINDING		181		181		Dihydroxyacetone phosphate; via amide nitrogen (By similarity)		G

Size range:	286-292 amino acids
Related UniRules:	MF_01294 (GATY)
Template:	P0AB74 (KBAY_ECOLI); Q9KIP8 (KBAY_ECOLX): [Recover all]
Scope:	Bacteria; Enterobacteriales
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	Subunit kbaZ is described in MF_01295. In some enterobacteria, a second D-tagatose-1,6-bisphosphate aldolase is present: gatYZ (MF_01294 and MF_01296).