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HAMAP annotation rule: MF_01291

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General rule information

Accession MF_01291
Dates 3-OCT-2008 (Created)
25-NOV-2009 (Last updated, Version 4)
Data class Protein
Predictors HAMAP; MF_01291; [distribution of match scores in UniProtKB];[seed alignment for MF_01291]
Names KDGluc_aldolase


Propagated annotation

Identifier, protein and gene names
Identifier GARL
Protein name
RecName: Full=5-keto-4-deoxy-D-glucarate aldolase;
Short=KDGluc aldolase;
Short=KDGlucA;
EC=4.1.2.20;
AltName: Full=5-dehydro-4-deoxy-D-glucarate aldolase;
AltName: Full=2-keto-3-deoxy-D-glucarate aldolase;
AltName: Full=2-dehydro-3-deoxy-D-glucarate aldolase;
AltName: Full=Alpha-keto-beta-deoxy-D-glucarate aldolase;
Gene name garL
Comments
FUNCTION: Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde (By similarity).
CATALYTIC ACTIVITY: 5-dehydro-4-deoxy-D-glucarate = pyruvate + tartronate semialdehyde.
CATALYTIC ACTIVITY: 2-dehydro-3-deoxy-D-glucarate = pyruvate + tartronate semialdehyde.

case <FTGroup:1>
COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
end case

PATHWAY: Carbohydrate acid metabolism; D-galactarate degradation; D-glycerate from D-galactarate: step 2/3.
SUBUNIT: Homohexamer; trimer of dimers (By similarity).
SIMILARITY: Belongs to the hpcH/hpaI aldolase family. KDGluc aldolase subfamily.
Cross-references
Pfam PF03328; HpcH_HpaI; 1;
TIGRFAMs TIGR03239; GarL; 1;
Keywords
Lyase.

case <FTGroup:1>
Magnesium, Metal-binding.
end case

Gene Ontology

case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case

GO:0008672; Molecular function: 2-dehydro-3-deoxyglucarate aldolase activity.
GO:0019394; Biological process: glucarate catabolic process.
GO:0019582; Biological process: D-galactarate catabolic process.
Features
From: GARL_ECOLI (P23522)
Key     From     To       Description   Condition   FTGroup
ACT_SITE     50     50       Proton acceptor (By similarity)   H  
 
METAL     153     153       Magnesium (By similarity)   E   1
METAL     179     179       Magnesium (By similarity)   D   1
 
BINDING     151     151       Substrate (By similarity)   Q  
BINDING     178     178       Substrate; via amide nitrogen (By similarity)   S  
BINDING     179     179       Substrate (By similarity)   D  
SITE     75     75       Transition state stabilizer (By similarity)   R  
SITE     89     89       Increases basicity of active site His (By similarity)   D  


Additional information

Size range: 256-257 amino acids
Related UniRules: None
Template: P23522 (GARL_ECOLI)
Scope: Bacteria; Enterobacteriales
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: None

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