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| HAMAP annotation rule: MF_01228 |
| Accession | MF_01228 |
| Dates | 30-OCT-2002 (Created) 25-NOV-2009 (Last updated, Version 28) |
| Data class | Protein |
| Predictors | HAMAP; MF_01228; [distribution of match scores in UniProtKB];[seed alignment for MF_01228] |
| Names | Met_tRNA_synth_type2 |
| Identifier | SYM |
| Protein name |
|
| Gene name | metG |
FUNCTION: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity).
CATALYTIC ACTIVITY: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
end case
case <Feature:PS50886>
SUBUNIT: Homodimer (By similarity).
end case
case not <Feature:PS50886> and <OC:Bacteria>
SUBUNIT: Monomer (By similarity).
end case
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.
end case
case not <FT:3>
SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.
end case
case <Feature:PS50886>
SIMILARITY: Contains 1 tRNA-binding domain.
end case
| Pfam |
PF00133; tRNA-synt_1; 1; |
| PRINTS |
PR01041; TRNASYNTHMET; 1; |
| TIGRFAMs |
TIGR00398; metG; 1; TIGR00399; metG_C_term; 1; |
| PROSITE |
PS00178; AA_TRNA_LIGASE_I; 1; PS50886; TRBD; 0-1; trigger=PRU00209; |
Cytoplasm,
Aminoacyl-tRNA synthetase,
Protein biosynthesis,
Ligase,
ATP-binding,
Nucleotide-binding.
case <Feature:PS50886>
end case
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
end case
GO:0005524; Molecular function: ATP binding.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
| From: SYM_THET8 (P23395) | ||||||||||
| Key | From | To | Description | Condition | FTGroup | |||||
| MOTIF | 12 | 22 | "HIGH" region | [YDFAS]-Y-x(5)-H-[LIV]-G-[SHN] | ||||||
| MOTIF | 297 | 301 | "KMSKS" region | K-[MI]-[SG]-K-[ST] | ||||||
| METAL | 127 | 127 | Zinc (By similarity) | C | ||||||
| METAL | 130 | 130 | Zinc (By similarity) | C | ||||||
| METAL | 144 | 144 | Zinc (By similarity) | C | ||||||
| METAL | 147 | 147 | Zinc (By similarity) | [HC] | ||||||
case not <FT:6>
| From: SYM_SYNY3 (Q55729) | ||||||||||
| Key | From | To | Description | Condition | FTGroup | |||||
| METAL | 152 | 152 | Zinc (By similarity) | [HC] | ||||||
end case
case not <FT:7>
| From: SYM_MYCGE (P47267) | ||||||||||
| Key | From | To | Description | Condition | FTGroup | |||||
| METAL | 147 | 147 | Zinc (By similarity) | [HC] | ||||||
end case
| Size range: | 497-683 amino acids |
| Related UniRules: | MF_00098 (SYM) |
| Template: | P23395 (SYM_THET8); O05593 (SYM_MYCTU); P23920 (SYM_BACST): [Recover all] |
| Scope: | Bacteria |
| Fusion: | Nter: None; Cter: None |
| Duplicate: | in CLOPE |
| Plasmid encoded: | None |
| Comments: | Subfamily 2a links 1 zinc ion ion (shown in THET8) whereas subfamily 2b does not (shown in MYCTU). Possible wrong starts in AQUAE and SYNY3. |
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