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HAMAP annotation rule: MF_01168

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General rule information

Accession MF_01168
Dates 8-DEC-2005 (Created)
25-NOV-2009 (Last updated, Version 8)
Data class Protein
Predictors HAMAP; MF_01168; [distribution of match scores in UniProtKB];[seed alignment for MF_01168]
Names AGOG


Propagated annotation

Identifier, protein and gene names
Identifier AGOG
Protein name
RecName: Full=N-glycosylase/DNA lyase;
AltName: Full=AGOG;
AltName: Full=8-oxoguanine DNA glycosylase;
EC=3.2.2.-;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase;
Short=AP lyase;
EC=4.2.99.18;
Comments
FUNCTION: DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates (By similarity).
CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
DOMAIN: Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).
SIMILARITY: Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.
Cross-references
Pfam PF09171; DUF1886; 1;
PIRSF PIRSF008955; AGOG; 1;
Keywords
DNA damage, DNA excision, DNA repair, Hydrolase, Lyase.
Gene Ontology
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) lyase activity.
GO:0000702; Molecular function: oxidized base lesion DNA N-glycosylase activity.
GO:0006284; Biological process: base-excision repair.
GO:0006281; Biological process: DNA repair.
Features
From: AGOG_PYRAE (Q8ZVK6)
Key     From     To       Description   Condition   FTGroup
ACT_SITE     140     140       Schiff-base intermediate with DNA (By similarity)   K  
ACT_SITE     172     172       Potential   D  
BINDING     31     31       8-oxoguanine (By similarity)   Q  
BINDING     58     58       8-oxoguanine; via carbonyl oxygen (By similarity)   S  
BINDING     69     69       8-oxoguanine (By similarity)   W  
BINDING     144     144       8-oxoguanine (By similarity)   F  
BINDING     170     170       8-oxoguanine; via carbonyl oxygen (By similarity)   P  
BINDING     218     218       8-oxoguanine (By similarity)   D  
BINDING     222     222       8-oxoguanine (By similarity)   W  
REGION     125     184       Helix-hairpin-helix      


Additional information

Size range: 216-291 amino acids
Related UniRules: None
Template: Q8ZVK6 (AGOG_PYRAE)
Scope: Archaea
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: None

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