ExPASy Proteomics Server

Accession	MF_01026
Dates	17-JUL-2002 (Created) 25-NOV-2009 (Last updated, Version 32)

Data class

Protein

Predictors

HAMAP; MF_01026; [distribution of match scores in UniProtKB];[seed alignment for MF_01026]

Names

LeuC_type1

Identifier

LEUC

Protein name

RecName: Full=3-isopropylmalate dehydratase large subunit; EC=4.2.1.33; AltName: Full=Isopropylmalate isomerase; AltName: Full=Alpha-IPM isomerase; Short=IPMI;

Gene name

leuC

FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity).

CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H(2)O.

CATALYTIC ACTIVITY: (2S)-2-isopropylmaleate + H(2)O = (2S)-2-isopropylmalate.

COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.

SUBUNIT: Heterodimer of leuC and leuD (By similarity).

SIMILARITY: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.

Pfam	PF00330; Aconitase; 1;
PRINTS	PR00415; ACONITASE; 1;
TIGRFAMs	TIGR00170; leuC; 1;
PROSITE	PS00450; ACONITASE_1; 1; PS01244; ACONITASE_2; 1;

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis, Lyase, Metal-binding, Iron, Iron-sulfur, 4Fe-4S.

GO:0003861; Molecular function: 3-isopropylmalate dehydratase activity.
GO:0009098; Biological process: leucine biosynthetic process.

From: LEUC_ECOLI (P0A6A6)
Key		From		To		Description		Condition		FTGroup
METAL		347		347		Iron-sulfur (4Fe-4S) (By similarity)		C
METAL		407		407		Iron-sulfur (4Fe-4S) (By similarity)		C
METAL		410		410		Iron-sulfur (4Fe-4S) (By similarity)		C

Size range:	455-486 amino acids
Related UniRules:	MF_01027 (LEUC)
Template:	P0A6A6 (LEUC_ECOLI); P15717 (LEUC1_SALTY): [Recover all]
Scope:	Bacteria Archaea; Halobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in BRAJA, MANSM, SALCH, SALTY
Plasmid encoded:	in BUCAI, BUCAI, BUCAP, BUCDN, BUCML, BUCRP, BUCTS, BUCUA, BUCUD, BUCUE, BUCUH, BUCUM, BUCUN, BUCUO, BUCUS