ExPASy Proteomics Server

Accession	MF_01024
Dates	20-MAR-2003 (Created) 18-JAN-2010 (Last updated, Version 22)

Data class

Protein

Predictors

HAMAP; MF_01024; [distribution of match scores in UniProtKB];[seed alignment for MF_01024]

Names

HisD

Identifier

HISX

Protein name

RecName: Full=Histidinol dehydrogenase; Short=HDH; EC=1.1.1.23;

Gene name

hisD

FUNCTION: Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine (By similarity).

CATALYTIC ACTIVITY: L-histidinol + H(2)O + 2 NAD(+) = L-histidine + 2 NADH.

COFACTOR: Binds 1 zinc ion per subunit (By similarity).

PATHWAY: Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the histidinol dehydrogenase family.

Pfam	PF00815; Histidinol_dh; 1;
PRINTS	PR00083; HOLDHDRGNASE; 1;
TIGRFAMs	TIGR00069; hisD; 1;
PROSITE	PS00611; HISOL_DEHYDROGENASE; 1;

Amino-acid biosynthesis, Histidine biosynthesis, Oxidoreductase, NAD.

Metal-binding, Zinc.

GO:0004399; Molecular function: histidinol dehydrogenase activity.

GO:0008270; Molecular function: zinc ion binding.

GO:0000105; Biological process: histidine biosynthetic process.

From: HISX_ECOLI (P06988)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		326		326		Proton acceptor (By similarity)		E
ACT_SITE		327		327		Proton acceptor (By similarity)		H
METAL		259		259		Zinc (By similarity)		[EQ]		1
METAL		262		262		Zinc (By similarity)		H		1
METAL		360		360		Zinc (By similarity)		D		1
METAL		419		419		Zinc (By similarity)		H		1
BINDING		130		130		NAD (By similarity)		Y		2
BINDING		188		188		NAD (By similarity)		Q		2
BINDING		211		211		NAD (By similarity)		N		2
BINDING		237		237		Substrate (By similarity)		[ST]		3
BINDING		259		259		Substrate (By similarity)		Q		3
BINDING		262		262		Substrate (By similarity)		H		3
BINDING		327		327		Substrate (By similarity)		H		3
BINDING		360		360		Substrate (By similarity)		D		3
BINDING		414		414		Substrate (By similarity)		E		3
BINDING		419		419		Substrate (By similarity)		H		3

Size range:	368-480 amino acids
Related UniRules:	None
Template:	P06988 (HISX_ECOLI); P10370 (HISX_SALTY): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: MF_01023 (hisC)
Duplicate:	in ANASP, ANAVT, BACSK, COLP3, PELUB, RALEJ, RHILO, RHIME, RHOS4, SILPO, SYNY3
Plasmid encoded:	in RHIME
Comments:	Longer N-terminal in the second copy of RHILO; not shown in alignment and not taken into account in size range. Zinc-binding site problem in SYNY3, in the second copy of RHIME and in the third copy of RHILO; sequences not included in alignment. Divergent second copy in SYNY3; not shown in alignment and not taken into account in size range. PHOLL is a fusion between hisC and hisD. As other Enterobacteriaceae do not have such a fusion, this could be a sequencing artefact.