ExPASy Proteomics Server

Accession	MF_01012
Dates	19-APR-2002 (Created) 25-NOV-2009 (Last updated, Version 16)

Data class

Protein

Predictors

HAMAP; MF_01012; [distribution of match scores in UniProtKB];[seed alignment for MF_01012]

Names

23SrRNA_methyltr_RumB

Identifier

RUMB

Protein name

RecName: Full=23S rRNA (uracil-5-)-methyltransferase rumB; EC=2.1.1.-; AltName: Full=23S rRNA(M-5-U747)-methyltransferase;

Gene name

rumB

FUNCTION: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA (By similarity).

CATALYTIC ACTIVITY: S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing thymine.

SIMILARITY: Belongs to the RNA M5U methyltransferase family. RumB subfamily.

Pfam	PF05958; tRNA_U5-meth_tr; 1;
PROSITE	PS01230; TRMA_1; 1; PS01231; TRMA_2; 1;
TIGRFAMs	TIGR02085; meth_trns_rumB; 1;

Methyltransferase, rRNA processing, S-adenosyl-L-methionine, Transferase.

4Fe-4S, Iron, Iron-sulfur, Metal-binding.

GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.

GO:0005506; Molecular function: iron ion binding.

GO:0006364; Biological process: rRNA processing.

From: RUMB_ECOLI (P75817)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		334		334		Nucleophile (By similarity)		C
METAL		3		3		Iron-sulfur (4Fe-4S) (By similarity)		C		1
METAL		11		11		Iron-sulfur (4Fe-4S) (By similarity)		C		1
METAL		14		14		Iron-sulfur (4Fe-4S) (By similarity)		C		1
METAL		87		87		Iron-sulfur (4Fe-4S) (By similarity)		C		1
BINDING		212		212		S-adenosyl-L-methionine (By similarity)		Q
BINDING		241		241		S-adenosyl-L-methionine; via carbonyl oxygen (By similarity)		F
BINDING		262		262		S-adenosyl-L-methionine (By similarity)		E
BINDING		307		307		S-adenosyl-L-methionine (By similarity)		N

Size range:	375-392 amino acids
Related UniRules:	None
Template:	P75817 (RUMB_ECOLI); P55135 (RUMA_ECOLI): [Recover all]
Scope:	Bacteria; Gammaproteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None