ExPASy Proteomics Server

Accession	MF_00817
Dates	8-NOV-2005 (Created) 25-NOV-2009 (Last updated, Version 15)

Data class

Protein

Predictors

HAMAP; MF_00817; [distribution of match scores in UniProtKB];[seed alignment for MF_00817]

Names

QueF_type2

Identifier

QUEF

Protein name

RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase; EC=1.7.1.13; AltName: Full=7-cyano-7-carbaguanine reductase; AltName: Full=PreQ(0) reductase; AltName: Full=NADPH-dependent nitrile oxidoreductase;

Gene name

queF

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).

CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH.

PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Pfam	PF01227; GTP_cyclohydroI; 1;

Cytoplasm, NADP, Oxidoreductase, Queuosine biosynthesis.

GO:0046857; Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor.
GO:0008616; Biological process: queuosine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Size range:	262-289 amino acids
Related UniRules:	MF_00818 (QUEF)
Template:	Q46920 (QUEF_ECOLI)
Scope:	Bacteria; Proteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None