Search for
You are here: ExPASy CH  > Databases  > Around UniProtKB  > HAMAP

Home Proteomes Families Documents Downloads Links
HAMAP annotation rule: MF_00782

Send feedback new

General rule information

Accession MF_00782
Dates 4-MAY-2004 (Created)
25-NOV-2009 (Last updated, Version 16)
Data class Protein
Predictors HAMAP; MF_00782; [distribution of match scores in UniProtKB];[seed alignment for MF_00782]
Names Glut_biosynth


Propagated annotation

Identifier, protein and gene names
Identifier GSHAB
Protein name
RecName: Full=Glutathione biosynthesis bifunctional protein gshAB;
AltName: Full=Gamma-GCS-GS;
Short=GCS-GS;
RecName: Full=Glutamate--cysteine ligase;
EC=6.3.2.2;
AltName: Full=Gamma-glutamylcysteine synthetase;
AltName: Full=Gamma-ECS;
Short=GCS;
RecName: Full=Glutathione synthetase;
EC=6.3.2.3;
AltName: Full=Glutathione synthase;
AltName: Full=GSH synthetase;
Short=GSH-S;
Short=GSHase;
Short=GS;
Gene name gshAB, gshF
Comments
FUNCTION: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine (By similarity).
CATALYTIC ACTIVITY: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.
CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.
PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
SUBUNIT: Monomer (By similarity).
SIMILARITY: In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
Cross-references
Pfam PF04262; Glu_cys_ligase; 1;
TIGRFAMs TIGR01435; glu_cys_lig_rel; 1;
PROSITE PS50975; ATP_GRASP; 1; trigger=PRU00409;
Keywords
ATP-binding, Glutathione biosynthesis, Ligase, Multifunctional enzyme, Nucleotide-binding.
Gene Ontology
GO:0005524; Molecular function: ATP binding.
GO:0004363; Molecular function: glutathione synthase activity.
GO:0004357; Molecular function: glutamate-cysteine ligase activity.
GO:0006750; Biological process: glutathione biosynthetic process.
Features
From: GSHAB_STRA5 (Q8DXM9)
Key     From     To       Description   Condition   FTGroup
REGION     Nter     333       Glutamate--cysteine ligase      


Additional information

Size range: 750-779 amino acids
Related UniRules: MF_00578 (GSH1)
Template: Q8DXM9 (GSHAB_STRA5)
Scope: Bacteria; Firmicutes
Bacteria; Proteobacteria
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: None

View rule in raw text format (no links)



UniProtKB rule member sequences