ExPASy Proteomics Server

Accession	MF_00743
Dates	4-DEC-2003 (Created) 25-NOV-2009 (Last updated, Version 20)

Data class

Protein

Predictors

HAMAP; MF_00743; [distribution of match scores in UniProtKB];[seed alignment for MF_00743]

Names

FumaraseC

Identifier

FUMC

Protein name

RecName: Full=Fumarate hydratase class II; Short=Fumarase C; EC=4.2.1.2;

Gene name

fumC

CATALYTIC ACTIVITY: (S)-malate = fumarate + H(2)O.

PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.

SUBUNIT: Homotetramer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

MISCELLANEOUS: There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors (By similarity).

SIMILARITY: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Pfam	PF10415; FumaraseC_C; 1; PF00206; Lyase_1; 1;
PRINTS	PR00149; FUMRATELYASE; 1;
TIGRFAMs	TIGR00979; fumC_II; 1;
PROSITE	PS00163; FUMARATE_LYASES; 1;

Cytoplasm, Lyase, Tricarboxylic acid cycle.

GO:0004333; Molecular function: fumarate hydratase activity.
GO:0006099; Biological process: tricarboxylic acid cycle.
GO:0005737; Cellular component: cytoplasm.

From: FUMC_ECOLI (P05042)
Key		From		To		Description		Condition		FTGroup
REGION		129		132		B site (By similarity)		[HN]-P-N-D
REGION		139		141		Substrate binding (By similarity)		S-[ST]-N
BINDING		100		100		Substrate (By similarity)		T

Size range:	438-484 amino acids
Related UniRules:	None
Template:	P05042 (FUMC_ECOLI); P39461 (FUMC_SULSO): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in BRAJA, PSEAE
Plasmid encoded:	in RALSO