ExPASy Proteomics Server

Accession	MF_00536
Dates	30-JAN-2002 (Created) 25-NOV-2009 (Last updated, Version 23)

Data class

Protein

Predictors

HAMAP; MF_00536; [distribution of match scores in UniProtKB];[seed alignment for MF_00536]

Names

PdxA

Identifier

PDXA

Protein name

RecName: Full=4-hydroxythreonine-4-phosphate dehydrogenase; EC=1.1.1.262; AltName: Full=4-(phosphohydroxy)-L-threonine dehydrogenase;

Gene name

pdxA

FUNCTION: Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) (By similarity).

CATALYTIC ACTIVITY: 4-(phosphonooxy)-L-threonine + NAD(+) = (2S)-2-amino-3-oxo-4-phosphonooxybutanoate + NADH.

COFACTOR: Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt (By similarity).

COFACTOR: Binds 1 divalent metal cation per subunit (By similarity).

PATHWAY: Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5.

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

MISCELLANEOUS: The active site is located at the dimer interface (By similarity).

SIMILARITY: Belongs to the pdxA family.

Pfam	PF04166; PdxA; 1;
TIGRFAMs	TIGR00557; pdxA; 1;

Cytoplasm, Pyridoxine biosynthesis, Oxidoreductase, NAD, NADP, Metal-binding.

Cobalt, Magnesium, Zinc.

GO:0050570; Molecular function: 4-hydroxythreonine-4-phosphate dehydrogenase activity.
GO:0046872; Molecular function: metal ion binding.
GO:0042823; Biological process: pyridoxal phosphate biosynthetic process.
GO:0008615; Biological process: pyridoxine biosynthetic process.

GO:0050897; Molecular function: cobalt ion binding.
GO:0008270; Molecular function: zinc ion binding.
GO:0000287; Molecular function: magnesium ion binding.

GO:0005737; Cellular component: cytoplasm.

From: PDXA_ECOLI (P19624)
Key		From		To		Description		Condition		FTGroup
METAL		166		166		Divalent metal cation; shared with dimeric partner (By similarity)		H
METAL		211		211		Divalent metal cation; shared with dimeric partner (By similarity)		H
METAL		266		266		Divalent metal cation; shared with dimeric partner (By similarity)		H
BINDING (Optional)		136		136		Substrate (By similarity)		H
BINDING (Optional)		137		137		Substrate (By similarity)		[TS]
BINDING		274		274		Substrate (By similarity)		K
BINDING (Optional)		283		283		Substrate (By similarity)		N
BINDING (Optional)		292		292		Substrate (By similarity)		R

Size range:	307-364 amino acids
Related UniRules:	None
Template:	P19624 (PDXA_ECOLI); P58717 (PDXA1_SALTY); Q9I5U4 (PDXA1_PSEAE); P58718 (PDXA2_SALTY): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in PSEAE, RHIME, SALTI, SALTY
Plasmid encoded:	in RHIME, SPHAR