ExPASy Proteomics Server

Accession	MF_00419
Dates	25-JUN-2001 (Created) 25-NOV-2009 (Last updated, Version 35)

Data class

Protein

Predictors

HAMAP; MF_00419; [distribution of match scores in UniProtKB];[seed alignment for MF_00419]

Names

PurL_1

Identifier

PUR4

Protein name

RecName: Full=Phosphoribosylformylglycinamidine synthase; Short=FGAM synthase; Short=FGAMS; EC=6.3.5.3; AltName: Full=Formylglycinamide ribotide amidotransferase; Short=FGARAT; AltName: Full=Formylglycinamide ribotide synthetase;

Gene name

purL

CATALYTIC ACTIVITY: ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: In the N-terminal section; belongs to the FGAMS family.

SIMILARITY: Contains 1 glutamine amidotransferase type-1 domain.

Pfam	PF00586; AIRS; 1;
PROSITE	PS51273; GATASE_TYPE_1; 1; trigger=PRU00605;

Cytoplasm, Purine biosynthesis, Ligase, ATP-binding, Nucleotide-binding, Glutamine amidotransferase.

GO:0005524; Molecular function: ATP binding.
GO:0004642; Molecular function: phosphoribosylformylglycinamidine synthase activity.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: PUR4_ECOLI (P15254)
Key		From		To		Description		Condition		FTGroup
NP_BIND		307		318		ATP (Potential)		G-A-[SA]-T-G-[SAVI]-G-G-E-I-R-D
ACT_SITE		1135		1135		Nucleophile (By similarity)		C
ACT_SITE		1260		1260		By similarity		H
ACT_SITE		1262		1262		By similarity		E

Size range:	1293-1369 amino acids
Related UniRules:	MF_00420 (PURL); MF_00421 (PURQ)
Template:	P15254 (PUR4_ECOLI)
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None