ExPASy Proteomics Server

Accession	MF_00220
Dates	1-JUN-2001 (Created) 25-NOV-2009 (Last updated, Version 31)

Data class

Protein

Predictors

HAMAP; MF_00220_B; [distribution of match scores in UniProtKB];[seed alignment for MF_00220_B]

Predictors

HAMAP; MF_00220_A; [distribution of match scores in UniProtKB];[seed alignment for MF_00220_A]

Names

PyrC_type2

Identifier

PYRC

Protein name

RecName: Full=Dihydroorotase; Short=DHOase; EC=3.5.2.3;

Gene name

pyrC

CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.

COFACTOR: Binds 2 zinc ions per subunit (By similarity).

PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the DHOase family. Type 2 subfamily.

Pfam	PF01979; Amidohydro_1; 1;
TIGRFAMs	TIGR00857; pyrC_multi; 1;
PROSITE	PS00482; DIHYDROOROTASE_1; 1; PS00483; DIHYDROOROTASE_2; 1;

Pyrimidine biosynthesis, Hydrolase, Metal-binding, Zinc.

GO:0004151; Molecular function: dihydroorotase activity.
GO:0008270; Molecular function: zinc ion binding.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

From: PYRC_BACSU (P25995)
Key		From		To		Description		Condition		FTGroup
METAL		59		59		Zinc 1 (By similarity)		H
METAL		61		61		Zinc 1 (By similarity)		H
METAL		141		141		Zinc 1; via carbamate group (By similarity)		K
METAL		141		141		Zinc 2; via carbamate group (By similarity)		K
METAL		178		178		Zinc 2 (By similarity)		H
METAL		231		231		Zinc 2 (By similarity)		H
METAL		304		304		Zinc 1 (By similarity)		D
MOD_RES		141		141		N6-carboxylysine (By similarity)		K

From: PYRC_PYRAB (Q9UXV6)
Key		From		To		Description		Condition		FTGroup
METAL		57		57		Zinc 1 (By similarity)		H
METAL		59		59		Zinc 1 (By similarity)		H
METAL		130		130		Zinc 1; via carbamate group (By similarity)		K
METAL		130		130		Zinc 2; via carbamate group (By similarity)		K
METAL		164		164		Zinc 2 (By similarity)		H
METAL		204		204		Zinc 2 (By similarity)		H
METAL		272		272		Zinc 1 (By similarity)		D
MOD_RES		130		130		N6-carboxylysine (By similarity)		K

Size range:

376-447 amino acids

Size range:

383-467 amino acids

Related UniRules:	MF_00219 (PYRC)
Template:	P25995 (PYRC_BACSU)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None