ExPASy Proteomics Server

Accession	MF_00200
Dates	1-JUN-2001 (Created) 25-NOV-2009 (Last updated, Version 18)

Data class

Protein

Predictors

HAMAP; MF_00200; [distribution of match scores in UniProtKB];[seed alignment for MF_00200]

Names

RTC

Identifier

RTCA

Protein name

RecName: Full=Probable RNA 3'-terminal phosphate cyclase; Short=RNA-3'-phosphate cyclase; Short=RNA cyclase; EC=6.5.1.4;

Gene name

rtcA

FUNCTION: Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing (By similarity).

CATALYTIC ACTIVITY: ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

SUBCELLULAR LOCATION: Cytoplasm (Potential).

SIMILARITY: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Pfam	PF01137; RTC; 1;
PROSITE	PS01287; RTC; 1;

ATP-binding, Cytoplasm, Ligase, Nucleotide-binding.

GO:0003963; Molecular function: RNA-3'-phosphate cyclase activity.
GO:0005737; Cellular component: cytoplasm.

From: RTCA_ECOLI (P46849)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		308		308		By similarity		H

Size range:	328-361 amino acids
Related UniRules:	None
Template:	P46849 (RTCA_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None