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HAMAP annotation rule: MF_00193

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General rule information

Accession MF_00193
Dates 1-JUN-2001 (Created)
25-NOV-2009 (Last updated, Version 25)
Data class Protein
Predictors HAMAP; MF_00193; [distribution of match scores in UniProtKB];[seed alignment for MF_00193]
Names NadE


Propagated annotation

Identifier, protein and gene names
Identifier NADE

case not <Feature:PS50263>
Protein name
RecName: Full=NH(3)-dependent NAD(+) synthetase;
EC=6.3.1.5;
end case


case <Feature:PS50263>
Protein name
RecName: Full=Probable glutamine-dependent NAD(+) synthetase;
EC=6.3.5.1;
AltName: Full=NAD(+) synthase [glutamine-hydrolysing];
end case

Gene name nadE
Comments

case not <Feature:PS50263>
CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+).
end case


case <Feature:PS50263>
FUNCTION: Can use both glutamine or ammonia as a nitrogen source (By similarity).
CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + L-glutamine + H(2)O = AMP + diphosphate + NAD(+) + L-glutamate.
end case

PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.

case not <Feature:PS50263>
SIMILARITY: Belongs to the NAD synthetase family.
end case


case <Feature:PS50263>
SIMILARITY: In the C-terminal section; belongs to the NAD synthetase family.
SIMILARITY: Contains 1 CN hydrolase domain.
end case

Cross-references
TIGRFAMs TIGR00552; nadE; 1;
PROSITE PS50263; CN_HYDROLASE; 0-1; trigger=PRU00054;
Keywords
Ligase, NAD, ATP-binding, Nucleotide-binding.
Gene Ontology
GO:0005524; Molecular function: ATP binding.

case not <Feature:PS50263>
GO:0008795; Molecular function: NAD+ synthase activity.
end case


case <Feature:PS50263>
GO:0003952; Molecular function: NAD+ synthase (glutamine-hydrolyzing) activity.
end case

GO:0009435; Biological process: NAD biosynthetic process.
Features
From: NADE_ECOLI (P18843)
Key     From     To       Description   Condition   FTGroup
NP_BIND     46     53       ATP (By similarity)   G-x-S-[GS]-G-x-D-S  
ACT_SITE     48     48       By similarity   S  


Additional information

Size range: 241-343 amino acids
Related UniRules: None
Template: P08164 (NADE_BACSU); P18843 (NADE_ECOLI); P0A5L6 (NADE_MYCTU); Q03638 (NADE_RHOCA): [Recover all]
Scope: Bacteria
Archaea
Fusion: Nter: None; Cter: None
Duplicate: in THEMA
Plasmid encoded: None

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UniProtKB rule member sequences