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|
| HAMAP annotation rule: MF_00185 |
| Accession |
MF_00185 |
| Dates |
1-JUN-2001 (Created)
25-NOV-2009 (Last updated, Version 15) |
| Protein name |
| RecName: |
Full=tRNA Delta(2)-isopentenylpyrophosphate transferase;
Short=IPP transferase;
EC=2.5.1.8; |
| AltName: |
Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
Short=IPTase;
Short=IPPT; |
| AltName: |
Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
Short=DMAPP:tRNA dimethylallyltransferase;
Short=DMATase; |
|
FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) (By similarity).
CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine.
COFACTOR: Magnesium (By similarity).
SUBUNIT: Monomer (By similarity).
SIMILARITY: Belongs to the IPP transferase family.
GO:0005524; Molecular function: ATP binding.
GO:0004811; Molecular function: tRNA isopentenyltransferase activity.
GO:0008033; Biological process: tRNA processing.
| From: MIAA_ECOLI (P16384) |
| Key |
|
From |
|
To |
|
Description |
|
Condition |
|
FTGroup |
| NP_BIND |
|
17 |
|
24 |
|
ATP (Potential) |
|
|
|
|
| REGION (Optional) |
|
19 |
|
24 |
|
Substrate binding (By similarity) |
|
[ST]-x-x-[GN]-K-[ST] |
|
|
| REGION (Optional) |
|
42 |
|
45 |
|
Interaction with substrate tRNA (By similarity) |
|
D-S-x-x |
|
|
| REGION (Optional) |
|
166 |
|
170 |
|
Interaction with substrate tRNA (By similarity) |
|
Q-R-x-x-R |
|
|
| REGION (Optional) |
|
247 |
|
252 |
|
Interaction with substrate tRNA (By similarity) |
|
R-C-V-G-Y-R |
|
|
| REGION (Optional) |
|
280 |
|
287 |
|
Interaction with substrate tRNA (By similarity) |
|
K-R-Q-I-T-W-L-R |
|
|
| SITE (Optional) |
|
108 |
|
108 |
|
Interaction with substrate tRNA (By similarity) |
|
[TS] |
|
|
| SITE (Optional) |
|
130 |
|
130 |
|
Interaction with substrate tRNA (By similarity) |
|
R |
|
|
| Size range: |
279-363 amino acids |
| Related UniRules: |
None |
| Template: |
P16384 (MIAA_ECOLI) |
| Scope: |
Bacteria |
| Fusion: |
Nter: None; Cter: None |
| Duplicate: |
in AZOPC, BACFN, BACFR, BACTN, BACV8, GEOBB, GEOLS, GEOSF, GEOUR, HAHCH, MYCMM, MYCUA, PARD8, PELCD, PELPD, PORG3, PORGI, SHESH, SYNAS, TREDE |
| Plasmid encoded: |
in THERP |
| Comments: |
Possible wrong start in a number of cases |
View rule in raw text format (no links)
