ExPASy Proteomics Server

Accession	MF_00156
Dates	1-JUN-2001 (Created) 25-NOV-2009 (Last updated, Version 22)

Data class

Protein

Predictors

HAMAP; MF_00156; [distribution of match scores in UniProtKB];[seed alignment for MF_00156]

Names

PanB

Identifier

PANB

Protein name

RecName: Full=3-methyl-2-oxobutanoate hydroxymethyltransferase; EC=2.1.2.11; AltName: Full=Ketopantoate hydroxymethyltransferase; Short=KPHMT;

Gene name

panB

FUNCTION: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate (By similarity).

CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H(2)O = tetrahydrofolate + 2-dehydropantoate.

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

PATHWAY: Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.

SUBUNIT: Homodecamer; pentamer of dimers (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (Potential).

SIMILARITY: Belongs to the panB family.

Pfam	PF02548; Pantoate_transf; 1;
PRINTS	PR01415; ANKYRIN; 1;
TIGRFAMs	TIGR00222; panB; 1;

Cytoplasm, Magnesium, Metal-binding, Methyltransferase, Pantothenate biosynthesis, Transferase.

GO:0003864; Molecular function: 3-methyl-2-oxobutanoate hydroxymethyltransferase activity.
GO:0015940; Biological process: pantothenate biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: PANB_ECOLI (P31057)
Key		From		To		Description		Condition		FTGroup
REGION		45		46		Substrate binding (By similarity)
ACT_SITE		181		181		Proton acceptor (By similarity)		E
METAL		45		45		Magnesium (By similarity)		D
METAL		84		84		Magnesium (By similarity)		D
METAL		114		114		Magnesium (By similarity)		E
BINDING		84		84		Alpha-ketoisovalerate (By similarity)		D
BINDING		112		112		Alpha-ketoisovalerate (By similarity)		K

Size range:	257-326 amino acids
Related UniRules:	None
Template:	P0A5Q8 (PANB_MYCTU); P31057 (PANB_ECOLI); Q9X712 (PANB_CORGL); Q9JZW6 (PANB_NEIMB): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in BRAJA, BURCH, BURCM, BURS3, HAHCH, METPP, PSEAB, PSEAE, PSEE4, PSEF5, VIBF1, ZYMMO
Plasmid encoded:	in RHIEC, RHIL3, SILST