ExPASy Proteomics Server

Accession	MF_00116
Dates	1-JUN-2001 (Created) 25-NOV-2009 (Last updated, Version 21)

Data class

Protein

Predictors

HAMAP; MF_00116; [distribution of match scores in UniProtKB];[seed alignment for MF_00116]

Names

dUTPase_bact

Identifier

DUT

Protein name

RecName: Full=Deoxyuridine 5'-triphosphate nucleotidohydrolase; Short=dUTPase; EC=3.6.1.23; AltName: Full=dUTP pyrophosphatase;

Gene name

dut

FUNCTION: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA (By similarity).

CATALYTIC ACTIVITY: dUTP + H(2)O = dUMP + diphosphate.

COFACTOR: Magnesium (By similarity).

PATHWAY: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.

SUBUNIT: Homotrimer (By similarity).

SIMILARITY: Belongs to the dUTPase family.

Pfam	PF00692; dUTPase; 1;
TIGRFAMs	TIGR00576; dut; 1;

Hydrolase, Magnesium, Metal-binding, Nucleotide metabolism.

GO:0004170; Molecular function: dUTP diphosphatase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006220; Biological process: pyrimidine nucleotide metabolic process.

From: DUT_ECOLI (P06968)
Key		From		To		Description		Condition		FTGroup
REGION (Optional)		70		72		Substrate binding (By similarity)		R-S-[GS]
REGION (Optional)		87		89		Substrate binding (By similarity)		[TVL]-[IV]-D
BINDING (Optional)		83		83		Substrate (By similarity)		[NQ]

Key		From		To		Description		Condition		FTGroup
BINDING (Optional)		89		89		Substrate (By similarity)		D

Key		From		To		Description		Condition		FTGroup
BINDING (Optional)		97		97		Substrate; via amide nitrogen and carbonyl oxygen (By similarity)		[KM]

Size range:	139-185 amino acids
Related UniRules:	None
Template:	P06968 (DUT_ECOLI); P0A552 (DUT_MYCTU): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None