ExPASy Proteomics Server

Accession	MF_00086
Dates	1-JUN-2001 (Created) 25-NOV-2009 (Last updated, Version 32)

Data class

Protein

Predictors

HAMAP; MF_00086; [distribution of match scores in UniProtKB];[seed alignment for MF_00086]

Names

S-AdoMet_synth1

Identifier

METK

Protein name

RecName: Full=S-adenosylmethionine synthetase; EC=2.5.1.6; AltName: Full=Methionine adenosyltransferase; AltName: Full=AdoMet synthetase; AltName: Full=MAT;

Gene name

metK

FUNCTION: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme (By similarity).

CATALYTIC ACTIVITY: ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine.

COFACTOR: Binds 2 divalent ions per subunit. Magnesium or cobalt (By similarity).

COFACTOR: Binds 1 potassium ion per subunit (By similarity).

PATHWAY: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.

SUBUNIT: Homotetramer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the AdoMet synthetase family.

Pfam	PF02773; S-AdoMet_synt_C; 1; PF02772; S-AdoMet_synt_M; 1; PF00438; S-AdoMet_synt_N; 1;
PIRSF	PIRSF000497; MAT; 1;
TIGRFAMs	TIGR01034; metK; 1;
PROSITE	PS00376; ADOMET_SYNTHETASE_1; 1; PS00377; ADOMET_SYNTHETASE_2; 1;

Acetylation.

ATP-binding, Cobalt, Cytoplasm, Magnesium, Metal-binding, Nucleotide-binding, One-carbon metabolism, Potassium, Transferase.

GO:0005524; Molecular function: ATP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0004478; Molecular function: methionine adenosyltransferase activity.
GO:0006730; Biological process: one-carbon metabolic process.
GO:0005737; Cellular component: cytoplasm.

From: METK_ECOLI (P0A817)
Key		From		To		Description		Condition		FTGroup
NP_BIND		260		267		ATP (Potential)
METAL		17		17		Magnesium (By similarity)		D
METAL		43		43		Potassium (By similarity)		E
METAL		264		264		Potassium (By similarity)		S
METAL		272		272		Magnesium (By similarity)		D

Key		From		To		Description		Condition		FTGroup
MOD_RES		3		3		N6-acetyllysine (By similarity)		K

Size range:	376-430 amino acids
Related UniRules:	None
Template:	P0A817 (METK_ECOLI)
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in FRASC, RHOPB, RHORT
Plasmid encoded:	None