ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry O95150


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name TNF15_HUMAN
Primary accession number O95150
Secondary accession numbers Q3SX69 Q5VJK8 Q5VWH1 Q8NFE9
Integrated into Swiss-Prot on June 6, 2002
Sequence was last modified on May 20, 2008 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 74)
Name and origin of the protein
Protein name Tumor necrosis factor ligand superfamily member 15
Synonyms Vascular endothelial cell growth inhibitor
TNF ligand-related molecule 1
Contains Tumor necrosis factor ligand superfamily member 15, membrane form
Tumor necrosis factor ligand superfamily member 15, secreted form
Gene name
Name: TNFSF15
Synonyms: TL1, VEGI
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, AND TISSUE SPECIFICITY.
TISSUE=Umbilical vein;
PubMed=9872942 [NCBI, ExPASy, EBI, Israel, Japan]
Zhai Y., Ni J., Jiang G.-W., Lu J., Xing L., Lincoln C., Carter K.C., Janat F., Kozak D., Xu S., Rojas L., Aggarwal B.B., Ruben S., Li L.-Y., Gentz R., Yu G.-L.;
"VEGI, a novel cytokine of the tumor necrosis factor family, is an angiogenesis inhibitor that suppresses the growth of colon carcinomas in vivo.";
FASEB J. 13:181-189(1999).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INDUCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
PubMed=11923219 [NCBI, ExPASy, EBI, Israel, Japan]
Chew L.-J., Pan H., Yu J., Tian S., Huang W.-Q., Zhang J.Y., Pang S., Li L.-Y.;
"A novel secreted splice variant of vascular endothelial cell growth inhibitor.";
FASEB J. 16:742-744(2002).
[3]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF N-TERMINUS, FUNCTION, INTERACTION WITH TNFRSF25 AND TNFRSF6B, SUBCELLULAR LOCATION, PROTEOLYTIC PROCESSING, INDUCTION, AND TISSUE SPECIFICITY.
DOI=10.1016/S1074-7613(02)00283-2; PubMed=11911831 [NCBI, ExPASy, EBI, Israel, Japan]
Migone T.-S., Zhang J., Luo X., Zhuang L., Chen C., Hu B., Hong J.S., Perry J.W., Chen S.-F., Zhou J.X.H., Cho Y.H., Ullrich S., Kanakaraj P., Carrell J., Boyd E., Olsen H.S., Hu G., Pukac L., Liu D., Ni J., Kim S., Gentz R., Feng P., Moore P.A., Ruben S.M., Wei P.;
"TL1A is a TNF-like ligand for DR3 and TR6/DcR3 and functions as a T cell costimulator.";
Immunity 16:479-492(2002).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
TISSUE=Placenta;
DOI=10.1038/ng1285; PubMed=14702039 [NCBI, ExPASy, EBI, Israel, Japan]
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
"Complete sequencing and characterization of 21,243 full-length human cDNAs.";
Nat. Genet. 36:40-45(2004).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature02465; PubMed=15164053 [NCBI, ExPASy, EBI, Israel, Japan]
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.;
"DNA sequence and analysis of human chromosome 9.";
Nature 429:369-374(2004).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.;
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
[7]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[8]
IDENTIFICATION (ISOFORM 3), AND TISSUE SPECIFICITY.
DOI=10.1016/S0378-1119(97)00509-X; PubMed=9434163 [NCBI, ExPASy, EBI, Israel, Japan]
Tan K.B., Harrop J., Reddy M., Young P., Terrett J., Emery J., Moore G., Truneh A.;
"Characterization of a novel TNF-like ligand and recently described TNF ligand and TNF receptor superfamily genes and their constitutive and inducible expression in hematopoietic and non-hematopoietic cells.";
Gene 204:35-46(1997).
[9]
FUNCTION.
DOI=10.1038/sj.onc.1203059; PubMed=10597252 [NCBI, ExPASy, EBI, Israel, Japan]
Haridas V., Shrivastava A., Su J., Yu G.-L., Ni J., Liu D., Chen S.-F., Ni Y., Ruben S.M., Gentz R., Aggarwal B.B.;
"VEGI, a new member of the TNF family activates nuclear factor-kappa B and c-Jun N-terminal kinase and modulates cell growth.";
Oncogene 18:6496-6504(1999).
[10]
X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 71-251, DISULFIDE BOND, AND SUBUNIT.
DOI=10.1016/j.bbrc.2007.09.097; PubMed=17935696 [NCBI, ExPASy, EBI, Israel, Japan]
Jin T., Guo F., Kim S., Howard A., Zhang Y.-Z.;
"X-ray crystal structure of TNF ligand family member TL1A at 2.1A.";
Biochem. Biophys. Res. Commun. 364:1-6(2007).
Comments
  • FUNCTION: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.
  • SUBUNIT: Homotrimer.
  • SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein (Probable).
  • SUBCELLULAR LOCATION: Tumor necrosis factor ligand superfamily member 15, secreted form: Secreted.
  • ALTERNATIVE PRODUCTS: 3 named isoforms [FASTA] produced by alternative splicing.
    Name1
    SynonymsTL1A, VEGI-251
    Isoform IDO95150-1
    This is the isoform sequence displayed in this entry.
    Name2
    SynonymsVEGI-192
    Isoform IDO95150-2
    Features which should be applied to build the isoform sequence: VSP_033492.
    Name3
    SynonymsVEGI-174
    Isoform IDO95150-3
    Note: No experimental confirmation available.
    Features which should be applied to build the isoform sequence: VSP_033493, VSP_033494.
  • TISSUE SPECIFICITY: Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon.
  • INDUCTION: Up-regulated by IL-1-alpha and TNF-alpha.
  • SIMILARITY: Belongs to the tumor necrosis factor family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF039390; AAD08783.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY434464; AAR98573.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF520785; AAM77366.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK291642; BAF84331.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL390240; CAH73730.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL390240; CAH73731.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CH471090; EAW87431.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC069435; AAH69435.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC074940; AAH74940.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC074941; AAH74941.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC104462; AAI04463.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC104463; AAI04464.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00021380; -.
IPI00184879; -.
IPI00478864; -.
RefSeq NP_005109.2; -.
UniGene Hs.241382
3D structure databases
PDB
2O0O; X-ray; 3.00 A; A/B/C=72-251.[ExPASy / RCSB / EBI]
2QE3; X-ray; 2.50 A; A=72-251.[ExPASy / RCSB / EBI]
2RE9; X-ray; 2.10 A; A/B/C=72-251.[ExPASy / RCSB / EBI]
2RJK; X-ray; 2.30 A; A=72-251.[ExPASy / RCSB / EBI]
2RJL; X-ray; 2.05 A; A=72-251.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 2O0O; -.
2QE3; -.
2RE9; -.
2RJK; -.
2RJL; -.
ModBase O95150.
Organism-specific databases
GeneCards GC09M116591; -.
HGNC HGNC:11931; TNFSF15.
GenAtlas TNFSF15.
HPA HPA012948; -.
MIM 604052; gene. [NCBI / EBI]
PharmGKB PA36623; -.
Gene expression databases
ArrayExpress O95150; -.
Bgee O95150; -.
CleanEx HS_TNFSF15; -.
GermOnline ENSG00000181634; Homo sapiens.
Ontologies
GO
GO:0005615; Cellular component: extracellular space (inferred from electronic annotation from UniProtKB-KW).
GO:0005887; Cellular component: integral to plasma membrane (traceable author statement from ProtInc).
GO:0005125; Molecular function: cytokine activity (inferred from electronic annotation from UniProtKB-KW).
GO:0005164; Molecular function: tumor necrosis factor receptor binding (inferred from electronic annotation from InterPro).
GO:0006919; Biological process: activation of caspase activity (inferred from direct assay from MGI).
GO:0007250; Biological process: activation of NF-kappaB-inducing kinase activity (inferred from direct assay from MGI).
GO:0042107; Biological process: cytokine metabolic process (inferred from direct assay from MGI).
GO:0006955; Biological process: immune response (inferred from electronic annotation from InterPro).
GO:0001937; Biological process: negative regulation of endothelial cell proliferation (inferred from direct assay from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR006053; TNF_abc.
IPR006052; TNF_family.
IPR008983; Tumour_necrosis_fac-like.
Graphical view of domain structure.
Gene3D G3DSA:2.60.120.40; Tumour_necrosis_fac-like; 1.
Pfam PF00229; TNF; 1.
Pfam graphical view of domain structure.
PRINTS PR01234; TNECROSISFCT.
ProDom PD002012; TNF_subf; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00207; TNF; 1.
SMART graphical view of domain structure.
PROSITE PS50049; TNF_2; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE O95150; -.
Genome annotation databases
Ensembl ENSG00000181634; Homo sapiens. [Contig view]
GeneID 9966; -.
KEGG hsa:9966; -.
Phylogenomic databases
HOVERGEN O95150; -.
OMA O95150; CRPKARS.
Other
NextBio 37610; -.
SOURCE TNFSF15; Homo sapiens.
ProtoNet O95150.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Alternative splicing; Cytokine; Direct protein sequencing; Disulfide bond; Glycoprotein; Membrane; Polymorphism; Secreted; Signal-anchor; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   251  251     Tumor necrosis factor ligand superfamily member 15, membrane form. PRO_0000185505
CHAIN   72   251  180     Tumor necrosis factor ligand superfamily member 15, secreted form. PRO_0000333234
TOPO_DOM   1    35  35     Cytoplasmic (Potential). 
TRANSMEM   36    56  21     Signal-anchor for type II membrane protein (Potential). 
TOPO_DOM   57   251  195     Extracellular (Potential). 
SITE   71    72  2     Cleavage. 
CARBOHYD   133   133        N-linked (GlcNAc...) (Potential). 
CARBOHYD   229   229        N-linked (GlcNAc...) (Potential). 
DISULFID   162   202         
VAR_SEQ   1    85        MAEDLGLSFGETASVEMLPEHGSCRPKARSSSARWALTCC LVLLPFLAGLTTYLLVSQLRAQGEACVQFQALKGQEFAPS HQQVY -> MQLTKGRLHFSHPLSHTKHISPFVTD (in isoform 2). VSP_033492
VAR_SEQ   1    77        Missing (in isoform 3). VSP_033493
VAR_SEQ   78   100        APSHQQVYAPLRADGDKPRAHLT -> MRRFLSKVYSFPMRKLILFLVFP (in isoform 3). VSP_033494
VARIANT   110   110  1     F -> L (in dbSNP:rs16931745 [NCBI]). VAR_043130 [3D]
CONFLICT   34    34        R -> H (in Ref. 7; AAI04463). 
STRAND   34    36  3      
STRAND   43    46  4      
STRAND   50    52  3      
STRAND   56    58  3      
STRAND   61    63  3      
STRAND   68    81  14      
STRAND   98   107  10      
STRAND   109   111  3      
STRAND   120   124  5      
STRAND   128   143  16      
STRAND   148   154  7      
HELIX   156   158  3      
TURN   164   166  3      
STRAND   167   173  7      
Sequence information
Length: 251 AA [This is the length of the unprocessed precursor] Molecular weight: 28087 Da [This is the MW of the unprocessed precursor] CRC64: 65ED70E367E3446D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAEDLGLSFG ETASVEMLPE HGSCRPKARS SSARWALTCC LVLLPFLAGL TTYLLVSQLR 

        70         80         90        100        110        120 
AQGEACVQFQ ALKGQEFAPS HQQVYAPLRA DGDKPRAHLT VVRQTPTQHF KNQFPALHWE 

       130        140        150        160        170        180 
HELGLAFTKN RMNYTNKFLL IPESGDYFIY SQVTFRGMTS ECSEIRQAGR PNKPDSITVV 

       190        200        210        220        230        240 
ITKVTDSYPE PTQLLMGTKS VCEVGSNWFQ PIYLGAMFSL QEGDKLMVNV SDISLVDYTK 

       250 
EDKTFFGAFL L 

O95150 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!