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UniProtKB/Swiss-Prot entry P57730


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CAR18_HUMAN
Primary accession number P57730
Secondary accession number A2RRF8
Integrated into Swiss-Prot on January 11, 2001
Sequence was last modified on January 11, 2001 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 65)
Name and origin of the protein
Protein name Caspase recruitment domain-containing protein 18
Synonym Caspase-1 inhibitor Iceberg
Gene name
Name: CARD18
Synonyms: ICEBERG
ORFNames: UNQ5804/PRO19611
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CASP1, AND STRUCTURE BY NMR.
DOI=10.1016/S0092-8674(00)00108-2; PubMed=11051551 [NCBI, ExPASy, EBI, Israel, Japan]
Humke E.W., Shriver S.K., Starovasnik M.A., Fairbrother W.J., Dixit V.M.;
"ICEBERG: a novel inhibitor of interleukin-1beta generation.";
Cell 103:99-111(2000).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1101/gr.1293003; PubMed=12975309 [NCBI, ExPASy, EBI, Israel, Japan]
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.";
Genome Res. 13:2265-2270(2003).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
INTERACTION WITH CARD8.
DOI=10.1074/jbc.M107811200; PubMed=11821383 [NCBI, ExPASy, EBI, Israel, Japan]
Razmara M., Srinivasula S.M., Wang L., Poyet J.-L., Geddes B.J., DiStefano P.S., Bertin J., Alnemri E.S.;
"CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis.";
J. Biol. Chem. 277:13952-13958(2002).
Comments
  • FUNCTION: Inhibits generation of IL-1-beta by interacting with caspase-1 and preventing its association with RIP2.
  • SUBUNIT: Interacts with pro-CASP1. Interacts with CARD8.
  • TISSUE SPECIFICITY: Primarily expressed in the heart and placenta.
  • SIMILARITY: Contains 1 CARD domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF208005; AAG23528.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY358231; AAQ88598.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC131610; AAI31611.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00027987; -.
RefSeq NP_067546.1; -.
UniGene Hs.56279
3D structure databases
PDB
1DGN; NMR; -; A=2-90.[ExPASy / RCSB / EBI]
PDBsum 1DGN; -.
ModBase P57730.
PTM databases
PhosphoSite P57730; -.
Organism-specific databases
GeneCards GC11M104514; -.
H-InvDB HIX0035881; -.
HGNC HGNC:28861; CARD18.
GenAtlas CARD18.
MIM 605354; gene. [NCBI / EBI]
Gene expression databases
ArrayExpress P57730; -.
Bgee P57730; -.
CleanEx HS_CARD18; -.
GermOnline ENSG00000118168; Homo sapiens.
Ontologies
GO
GO:0005622; Cellular component: intracellular (inferred from electronic annotation from InterPro).
GO:0004197; Molecular function: cysteine-type endopeptidase activity (inferred from electronic annotation from InterPro).
GO:0004869; Molecular function: cysteine-type endopeptidase inhibitor activity (inferred from electronic annotation from UniProtKB-KW).
GO:0005515; Molecular function: protein binding (inferred from electronic annotation from InterPro).
GO:0006954; Biological process: inflammatory response (non-traceable author statement from UniProtKB).
GO:0006508; Biological process: proteolysis (inferred from electronic annotation from InterPro).
GO:0042981; Biological process: regulation of apoptosis (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001315; CARD.
IPR011029; DEATH-like.
IPR002398; Pept_C14_p45.
Graphical view of domain structure.
Gene3D G3DSA:1.10.533.10; DEATH_like; 1.
PANTHER PTHR10454; Pept_C14_p45; 1.
Pfam PF00619; CARD; 1.
Pfam graphical view of domain structure.
SMART SM00114; CARD; 1.
SMART graphical view of domain structure.
PROSITE PS50209; CARD; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE P57730; -.
Genome annotation databases
Ensembl ENSG00000118168; Homo sapiens. [Contig view]
GeneID 59082; -.
KEGG hsa:59082; -.
Phylogenomic databases
HOGENOM P57730; -.
HOVERGEN P57730; -.
Other
NextBio 65139; -.
SOURCE CARD18; Homo sapiens.
ProtoNet P57730.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Protease inhibitor; Thiol protease inhibitor.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom  To Length Description FTId
CHAIN   1   90  90     Caspase recruitment domain-containing protein 18. PRO_0000084145
DOMAIN   1   90  90     CARD. 
HELIX   5    8  4      
HELIX   10   14  5      
HELIX   19   32  14      
HELIX   37   44  8      
HELIX   50   63  14      
HELIX   66   79  14      
HELIX   83   87  5      
Sequence information
Length: 90 AA [This is the length of the unprocessed precursor] Molecular weight: 10138 Da [This is the MW of the unprocessed precursor] CRC64: 7E74C74263367101 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL 

        70         80         90 
VTGKGPKSCC KFIKHLCEED PQLASKMGLH 

P57730 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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