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UniProtKB/Swiss-Prot entry O43557


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name TNF14_HUMAN
Primary accession number O43557
Secondary accession numbers A8K7M2 O75476 Q8WVF8 Q96LD2
Integrated into Swiss-Prot on February 21, 2001
Sequence was last modified on June 1, 1998 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 92)
Name and origin of the protein
Protein name Tumor necrosis factor ligand superfamily member 14
Synonyms Herpesvirus entry mediator-ligand
HVEM-L
CD258 antigen
Contains Tumor necrosis factor ligand superfamily member 14, membrane form
Tumor necrosis factor ligand superfamily member 14, soluble form
Gene name
Name: TNFSF14
Synonyms: HVEML, LIGHT
ORFNames: UNQ391/PRO726
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
DOI=10.1016/S1074-7613(00)80455-0; PubMed=9462508 [NCBI, ExPASy, EBI, Israel, Japan]
Mauri D.N., Ebner R., Montgomery R.I., Kochel K.D., Cheung T.C., Yu G.-L., Ruben S., Murphy M., Eisenberg R.J., Cohen G.H., Spear P.G., Ware C.F.;
"LIGHT, a new member of the TNF superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator.";
Immunity 8:21-30(1998).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, AND VARIANT LYS-214.
TISSUE=Liver;
DOI=10.1074/jbc.273.42.27548; PubMed=9765287 [NCBI, ExPASy, EBI, Israel, Japan]
Harrop J.A., McDonnell P.C., Brigham-Burke M., Lyn S.D., Minton J., Tan K.B., Dede K., Spampanato J., Silverman C., Hensley P., DiPrinzio R., Emery J.G., Deen K., Eichman C., Chabot-Fletcher M., Truneh A., Young P.R.;
"Herpesvirus entry mediator ligand (HVEM-L), a novel ligand for HVEM/TR2, stimulates proliferation of T cells and inhibits HT29 cell growth.";
J. Biol. Chem. 273:27548-27556(1998).
[3]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND PROTEOLYTIC PROCESSING.
PubMed=11673523 [NCBI, ExPASy, EBI, Israel, Japan]
Granger S.W., Butrovich K.D., Houshmand P., Edwards W.R., Ware C.F.;
"Genomic characterization of LIGHT reveals linkage to an immune response locus on chromosome 19p13.3 and distinct isoforms generated by alternate splicing or proteolysis.";
J. Immunol. 167:5122-5128(2001).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
DOI=10.1101/gr.1293003; PubMed=12975309 [NCBI, ExPASy, EBI, Israel, Japan]
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.";
Genome Res. 13:2265-2270(2003).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT LYS-214.
TISSUE=Spleen;
DOI=10.1038/ng1285; PubMed=14702039 [NCBI, ExPASy, EBI, Israel, Japan]
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
"Complete sequencing and characterization of 21,243 full-length human cDNAs.";
Nat. Genet. 36:40-45(2004).
Comments
  • FUNCTION: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
  • SUBUNIT: Homotrimer.
  • INTERACTION:
    O95407:TNFRSF6B; NbExp=1; IntAct=EBI-524131, EBI-524171;
  • SUBCELLULAR LOCATION: Tumor necrosis factor ligand superfamily member 14, membrane form: Cell membrane; Single-pass type II membrane protein.
  • SUBCELLULAR LOCATION: Tumor necrosis factor ligand superfamily member 14, soluble form: Secreted.
  • SUBCELLULAR LOCATION: Isoform 2: Cytoplasm.
  • ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
    Name1
    Isoform IDO43557-1
    This is the isoform sequence displayed in this entry.
    Name2
    SynonymsLIGHT delta-TM
    Isoform IDO43557-2
    Features which should be applied to build the isoform sequence: VSP_006452.
  • TISSUE SPECIFICITY: Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
  • INDUCTION: Up-regulated after T-cell activation.
  • PTM: N-glycosylated.
  • PTM: The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
  • SIMILARITY: Belongs to the tumor necrosis factor family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF036581; AAC39563.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF064090; AAC25169.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY028261; AAK26160.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY358812; AAQ89171.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK292037; BAF84726.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00221341; -.
IPI00307531; -.
RefSeq NP_003798.2; -.
NP_742011.1; -.
UniGene Hs.129708
3D structure databases
HSSP P01375; 4TSV. [HSSP ENTRY / SWISS-3DIMAGE / PDB]
ModBase O43557.
Protein-protein interaction databases
IntAct O43557; 3.
Organism-specific databases
GeneCards GC19M006615; -.
HGNC HGNC:11930; TNFSF14.
GenAtlas TNFSF14.
MIM 604520; gene. [NCBI / EBI]
PharmGKB PA36622; -.
Gene expression databases
ArrayExpress O43557; -.
Bgee O43557; -.
CleanEx HS_TNFSF14; -.
GermOnline ENSG00000125735; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005615; Cellular component: extracellular space (inferred from electronic annotation from UniProtKB-KW).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0043027; Molecular function: caspase inhibitor activity (inferred from direct assay from UniProtKB).
GO:0005125; Molecular function: cytokine activity (inferred from electronic annotation from UniProtKB-KW).
GO:0005164; Molecular function: tumor necrosis factor receptor binding (inferred from electronic annotation from InterPro).
GO:0006955; Biological process: immune response (inferred from electronic annotation from InterPro).
GO:0006917; Biological process: induction of apoptosis (traceable author statement from ProtInc).
GO:0008588; Biological process: release of cytoplasmic sequestered NF-kappaB (inferred from direct assay from UniProtKB).
GO:0043029; Biological process: T cell homeostasis (non-traceable author statement from UniProtKB).
GO:0042098; Biological process: T cell proliferation (non-traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR006052; TNF_family.
IPR008983; Tumour_necrosis_fac-like.
Graphical view of domain structure.
Gene3D G3DSA:2.60.120.40; Tumour_necrosis_fac-like; 1.
Pfam PF00229; TNF; 1.
Pfam graphical view of domain structure.
ProDom PD002012; TNF_subf; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00207; TNF; 1.
SMART graphical view of domain structure.
PROSITE PS00251; TNF_1; FALSE_NEG.
PS50049; TNF_2; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
Ensembl ENSG00000125735; Homo sapiens. [Contig view]
GeneID 8740; -.
KEGG hsa:8740; -.
Phylogenomic databases
HOGENOM O43557; -.
HOVERGEN O43557; -.
Other
NextBio 32787; -.
SOURCE TNFSF14; Homo sapiens.
ProtoNet O43557.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Cell membrane; Cytokine; Cytoplasm; Disulfide bond; Glycoprotein; Membrane; Polymorphism; Secreted; Signal-anchor; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   240  240     Tumor necrosis factor ligand superfamily member 14, membrane form. PRO_0000034532
CHAIN   ?83   240        Tumor necrosis factor ligand superfamily member 14, soluble form. PRO_0000034533
TOPO_DOM   1    37  37     Cytoplasmic (Potential). 
TRANSMEM   38    58  21     Signal-anchor for type II membrane protein (Potential). 
TOPO_DOM   59   240  182     Extracellular (Potential). 
SITE   82    83  2     Cleavage (Potential). 
CARBOHYD   102   102        N-linked (GlcNAc...). 
DISULFID   154   187        Potential. 
VAR_SEQ   38    73        Missing (in isoform 2). VSP_006452
VARIANT   32    32  1     S -> L (in dbSNP:rs2291667 [NCBI]). VAR_027677 
VARIANT   120   120  1     L -> V (in dbSNP:rs17851606 [NCBI]). VAR_027678 
VARIANT   214   214  1     E -> K (in dbSNP:rs344560 [NCBI]). VAR_027679 
Sequence information
Length: 240 AA [This is the length of the unprocessed precursor] Molecular weight: 26351 Da [This is the MW of the unprocessed precursor] CRC64: 49D0BF67E1390B39 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEESVVRPSV FVVDGQTDIP FTRLGRSHRR QSCSVARVGL GLLLLLMGAG LAVQGWFLLQ 

        70         80         90        100        110        120 
LHWRLGEMVT RLPDGPAGSW EQLIQERRSH EVNPAAHLTG ANSSLTGSGG PLLWETQLGL 

       130        140        150        160        170        180 
AFLRGLSYHD GALVVTKAGY YYIYSKVQLG GVGCPLGLAS TITHGLYKRT PRYPEELELL 

       190        200        210        220        230        240 
VSQQSPCGRA TSSSRVWWDS SFLGGVVHLE AGEEVVVRVL DERLVRLRDG TRSYFGAFMV 

O43557 in FASTA format

View entry in raw text format (no links)
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