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UniProtKB/Swiss-Prot entry O00548


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DLL1_HUMAN
Primary accession number O00548
Secondary accession numbers Q9NU41 Q9UJV2
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on October 31, 2006 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 86)
Name and origin of the protein
Protein name Delta-like protein 1 [Precursor]
Synonyms Drosophila Delta homolog 1
Delta1
H-Delta-1
Gene name
Name: DLL1
ORFNames: UNQ146/PRO172
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=10079256 [NCBI, ExPASy, EBI, Israel, Japan]
Gray G.E., Mann R.S., Mitsiadis E., Henrique D., Carcangiu M.-L., Banks A., Leiman J., Ward D., Ish-Horowitz D., Artavanis-Tsakonas S.;
"Human ligands of the Notch receptor.";
Am. J. Pathol. 154:785-794(1999).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=10688816 [NCBI, ExPASy, EBI, Israel, Japan]
Han W., Ye Q., Moore M.A.S.;
"A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells.";
Blood 95:1616-1625(2000).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Oda T., Chandrasekharappa S.C.;
"Human Delta 1 gene sequence.";
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1101/gr.1293003; PubMed=12975309 [NCBI, ExPASy, EBI, Israel, Japan]
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.";
Genome Res. 13:2265-2270(2003).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature02055; PubMed=14574404 [NCBI, ExPASy, EBI, Israel, Japan]
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.;
"The DNA sequence and analysis of human chromosome 6.";
Nature 425:805-811(2003).
[6]
FUNCTION.
DOI=10.1084/jem.194.7.991; PubMed=11581320 [NCBI, ExPASy, EBI, Israel, Japan]
Jaleco A.C., Neves H., Hooijberg E., Gameiro P., Clode N., Haury M., Henrique D., Parreira L.;
"Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation.";
J. Exp. Med. 194:991-1001(2001).
Comments
  • FUNCTION: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • SUBUNIT: Interacts with Notch receptors.
  • SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
  • TISSUE SPECIFICITY: Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • PTM: Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation (By similarity).
  • SIMILARITY: Contains 1 DSL domain.
  • SIMILARITY: Contains 8 EGF-like domains.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF003522; AAB61286.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF196571; AAF05834.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF222310; AAG09716.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY358892; AAQ89251.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL078605; CAB89569.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_005609.3; -.
UniGene Hs.379912
3D structure databases
HSSP P00740; 1EDM. [HSSP ENTRY / PDB]
ModBase O00548.
PTM databases
PhosphoSite O00548; -.
Enzyme and pathway databases
Reactome REACT_299; Signaling by Notch.
Organism-specific databases
HGNC HGNC:2908; DLL1.
GenAtlas DLL1.
MIM 606582; gene. [NCBI / EBI]
PharmGKB PA27364; -.
GeneCards O00548.
Gene expression databases
ArrayExpress O00548; -.
CleanEx HS_DLL1; -.
GermOnline ENSG00000198719; Homo sapiens.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (non-traceable author statement from UniProtKB).
GO:0005887; Cellular component: integral to plasma membrane (non-traceable author statement from UniProtKB).
GO:0005112; Molecular function: Notch binding (inferred from physical interaction from UniProtKB).
GO:0001709; Biological process: cell fate determination (non-traceable author statement from UniProtKB).
GO:0030097; Biological process: hemopoiesis (non-traceable author statement from UniProtKB).
GO:0007219; Biological process: Notch signaling pathway (non-traceable author statement from UniProtKB).
GO:0030155; Biological process: regulation of cell adhesion (traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR001774; DSL.
IPR006210; EGF.
IPR000152; EGF-type_Asp/Asn_hydroxyl_CS.
IPR001438; EGF_2.
IPR000742; EGF_3.
IPR001881; EGF_Ca_bd.
IPR006209; EGF_like.
IPR013032; EGF_like_reg_CS.
IPR011651; Notch_ligand_N.
Graphical view of domain structure.
Pfam PF01414; DSL; 1.
PF00008; EGF; 6.
PF07657; MNNL; 1.
Pfam graphical view of domain structure.
PRINTS PR00010; EGFBLOOD.
SMART SM00051; DSL; 1.
SM00181; EGF; 4.
SM00179; EGF_CA; 4.
SMART graphical view of domain structure.
PROSITE PS00010; ASX_HYDROXYL; 3.
PS51051; DSL; 1.
PS00022; EGF_1; 8.
PS01186; EGF_2; 8.
PS50026; EGF_3; 7.
PS01187; EGF_CA; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS O00548.
Genome annotation databases
Ensembl ENSG00000198719; Homo sapiens. [Contig view]
GeneID 28514; -.
KEGG hsa:28514; -.
Phylogenomic databases
HOGENOM O00548; -.
HOVERGEN O00548; -.
Other
SOURCE DLL1; Homo sapiens.
ProtoNet O00548.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Developmental protein; Differentiation; EGF-like domain; Glycoprotein; Membrane; Notch signaling pathway; Repeat; Signal; Transmembrane; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    17  17     Potential. 
CHAIN   18   723  706     Delta-like protein 1. PRO_0000007506
TOPO_DOM   18   545  528     Extracellular (Potential). 
TRANSMEM   546   568  23     Potential. 
TOPO_DOM   569   723  155     Cytoplasmic (Potential). 
DOMAIN   177   221  45     DSL. 
DOMAIN   226   254  29     EGF-like 1. 
DOMAIN   257   285  29     EGF-like 2. 
DOMAIN   292   325  34     EGF-like 3. 
DOMAIN   332   363  32     EGF-like 4; calcium-binding (Potential). 
DOMAIN   370   402  33     EGF-like 5. 
DOMAIN   409   440  32     EGF-like 6. 
DOMAIN   447   478  32     EGF-like 7; calcium-binding (Potential). 
DOMAIN   485   516  32     EGF-like 8. 
CARBOHYD   477   477        N-linked (GlcNAc...) (Potential). 
DISULFID   226   237        By similarity. 
DISULFID   230   243        By similarity. 
DISULFID   245   254        By similarity. 
DISULFID   257   268        By similarity. 
DISULFID   263   274        By similarity. 
DISULFID   276   285        By similarity. 
DISULFID   292   304        By similarity. 
DISULFID   298   314        By similarity. 
DISULFID   316   325        By similarity. 
DISULFID   332   343        By similarity. 
DISULFID   337   352        By similarity. 
DISULFID   354   363        By similarity. 
DISULFID   370   381        By similarity. 
DISULFID   375   391        By similarity. 
DISULFID   393   402        By similarity. 
DISULFID   409   420        By similarity. 
DISULFID   414   429        By similarity. 
DISULFID   431   440        By similarity. 
DISULFID   447   458        By similarity. 
DISULFID   452   467        By similarity. 
DISULFID   469   478        By similarity. 
DISULFID   485   496        By similarity. 
DISULFID   490   505        By similarity. 
DISULFID   507   516        By similarity. 
CONFLICT   498   498        E -> Q (in Ref. 2; AAF05834). 
CONFLICT   502   502        R -> G (in Ref. 1; AAB61286, 2; AAF05834 and 3; AAG09716). 
CONFLICT   510   510        G -> S (in Ref. 2; AAF05834). 
Sequence information
Length: 723 AA [This is the length of the unprocessed precursor] Molecular weight: 78056 Da [This is the MW of the unprocessed precursor] CRC64: 094B8F235DFD899D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGSRCALALA VLSALLCQVW SSGVFELKLQ EFVNKKGLLG NRNCCRGGAG PPPCACRTFF 

        70         80         90        100        110        120 
RVCLKHYQAS VSPEPPCTYG SAVTPVLGVD SFSLPDGGGA DSAFSNPIRF PFGFTWPGTF 

       130        140        150        160        170        180 
SLIIEALHTD SPDDLATENP ERLISRLATQ RHLTVGEEWS QDLHSSGRTD LKYSYRFVCD 

       190        200        210        220        230        240 
EHYYGEGCSV FCRPRDDAFG HFTCGERGEK VCNPGWKGPY CTEPICLPGC DEQHGFCDKP 

       250        260        270        280        290        300 
GECKCRVGWQ GRYCDECIRY PGCLHGTCQQ PWQCNCQEGW GGLFCNQDLN YCTHHKPCKN 

       310        320        330        340        350        360 
GATCTNTGQG SYTCSCRPGY TGATCELGID ECDPSPCKNG GSCTDLENSY SCTCPPGFYG 

       370        380        390        400        410        420 
KICELSAMTC ADGPCFNGGR CSDSPDGGYS CRCPVGYSGF NCEKKIDYCS SSPCSNGAKC 

       430        440        450        460        470        480 
VDLGDAYLCR CQAGFSGRHC DDNVDDCASS PCANGGTCRD GVNDFSCTCP PGYTGRNCSA 

       490        500        510        520        530        540 
PVSRCEHAPC HNGATCHERG HRYVCECARG YGGPNCQFLL PELPPGPAVV DLTEKLEGQG 

       550        560        570        580        590        600 
GPFPWVAVCA GVILVLMLLL GCAAVVVCVR LRLQKHRPPA DPCRGETETM NNLANCQREK 

       610        620        630        640        650        660 
DISVSIIGAT QIKNTNKKAD FHGDHSADKN GFKARYPAVD YNLVQDLKGD DTAVRDAHSK 

       670        680        690        700        710        720 
RDTKCQPQGS SGEEKGTPTT LRGGEASERK RPDSGCSTSK DTKYQSVYVI SEEKDECVIA 


TEV 

O00548 in FASTA format

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