PeptideMass

Enter a UniProtKB protein identifier, ID (e.g. ALBU_HUMAN), or accession number, AC (e.g. P04406), or an amino acid sequence (e.g. 'SELVEGVIV'; you may specify post-translational modifications, but PLEASE read this document first!):

the fields. the cleavage of the protein.

The peptide masses are

with cysteines treated with: nothing (in reduced form) Iodoacetic acid Iodoacetamide 4-vinyl pyridene

with acrylamide adducts

with methionines oxidized

[M+H]⁺ or [M] or [M-H]^- .

average or monoisotopic.

Select an enzyme : Trypsin Trypsin (C-term to K/R, even before P) Trypsin (higher specificity) Trypsin/CNBr Lys C Lys N CNBr Arg C Asp N Asp N + N-terminal Glu Asp N / Lys C Asp N + N-terminal Glu / Lys C Glu C (bicarbonate) Glu C (phosphate) Glu C (phosphate) + Lys C Chymotrypsin (C-term to F/Y/W/M/L, not before P) Chymotrypsin (C-term to F/Y/W, not before P) Trypsin/Chymotrypsin (C-term to K/R/F/Y/W, not before P) Pepsin (pH 1.3) Pepsin (pH > 2) Proteinase K Thermolysin No cutting

Allow for 0 1 2 3 4 5 missed cleavages.
Display the peptides with a mass bigger than 0 500 750 1000 1250 1500 1750 and smaller than 3000 4000 5000 6000 7000 8000 unlimited Dalton

sorted by peptide masses or in chronological order in the protein.

For UniProtKB (Swiss-Prot/TrEMBL) entries only:
For each peptide display
all known post-translational modifications,
all database conflicts,
all variants (polymorphisms ),
all mRNA variants (due to alternative splicing, initiation or promoter usage).